EBV-DNA、T-钙黏蛋白和PCNA在鼻咽癌患者血清中的表达及其临床意义  

Expression and Clinical Significance of EBV-DNA,T-cadherin and PCNA in Serum of Patients with Nasopharyngeal Carcinoma

作　　者：林小梅[1] 宁春玉[1] 潘琦津[1] 张芸[1] LIN Xiao-mei;NING Chun-yu;PAN Qi-jin(Department of cancer prevention and treatment center,Guigang People's Hospital,Guangxi Guigang,537100)

机构地区：[1]广西贵港市人民医院肿瘤防治中心,广西贵港537100

出　　处：《医学临床研究》2020年第4期563-566,共4页Journal of Clinical Research

摘　　要：[目的]探讨EB病毒(EBV)-DNA、T-钙黏蛋白(T-cadherin)和增殖细胞核抗原(PCNA)在鼻咽癌患者血清中的表达及其临床意义.[方法]选取2013年1月至2015年12月在本院收治的150例晚期(Ⅲ~Ⅳa期)鼻咽癌患者作为鼻咽癌组,另外选取同期在本院诊治的50例慢性鼻炎(鼻炎组)和体检的健康志愿者50例(对照组).采用实时荧光定量PCR检测治疗前后EBV-DNA、T-cadherin mRNA和PCNA mRNA水平;随访2年,记录复发转移情况,并分析其与以上检测指标的关系.[结果]鼻咽癌组、鼻炎组和对照组三组在EBV-DNA阳性率、T-cadherin mRNA和PCNA mRNA比较差异有统计学意义(P<0.05);与治疗前比较,鼻咽癌组治疗后PCNA mRNA水平明显降低(P<0.05),T-cadherin mRNA明显升高(P<0.05).150例鼻咽癌患者中治疗敏感(TS)124例,TR者26例;治疗前,治疗抵抗(TR)者EBV-DNA阳性率和PCNA mR-NA均高于TS者,而则T-cadherin mRNA低于TS者,差异均具有统计学意义(P<0.05).150例鼻咽癌放疗后患者,其中35例出现复发转移,复发转移率23.33%,其中3例复发,32例远处转移.转移者EBV-DNA阳性率明显高于无复发转移者(P<0.05),无复发转移者T-cadherin mRNA高于复发者和转移者(P<0.05),无复发转移者PCNA mRNA低于复发者和转移者(P<0.05).[结论]EBV-DNA、T-cadherin和PCNA在晚期鼻咽癌患者放疗前后及放疗后复发转移中差异表达,有望成为疗效及预后评估的有效指标.[Objective]To investigate the expression and clinical significance of EB virus(EBV)-DNA,T-cadherin(T-cadherin)and proliferating nuclear antigen(PCNA)in the serum of patients with NPC.[Meth-ods]From January 2013 to December 2015,150 patients with advanced(Ⅲ~Ⅳ A)NPC were selected as NPC group,and 50 patients with chronic rhinitis(rhinitis group)and 50 healthy volunteers(control group)were selected as NPC group.The levels of EBV-DNA,T-cadherin mRNA and PCNA mRNA before and after treatment were detected by real time fluorescent quantitative PCR,and the recurrence and metastasis were recorded after 2 years of follow up,and the relationship between them and the above detection indexes was analyzed.[Results]There were statistically significant differences in EBV-DNA positive rate.T-cadherin mRNA and PCNA mRNA between the three groups of the nasopharyngeal carcinoma group,the rhinitis group and the control group(P<0.05);compared with the treatment before treatment,the PCNA mRNA level of the nasopharyngeal carcinoma group was significantly decreased(P<0.05)and the T-cadherin mRNA was significantly inereased(P<0.05).124 cases of treatment sensitivity(TS)and 26 cases of TR were found in 150 patients with nasopharyngeal carcinoma;before treatment,EBV-DNA positive rate and PCNA mRNA in TR patients were higher than those in TS patients,while T-cadherin mRNA was lower than that in TS patients,the difference was statistically significant(P<0.05).Of 150 patients with nasopharyngeal carcinoma after radiotherapy,35 of them had recurrence and metastasis.and the rate of recurrence and metastasis was 23.33% of which 3 had recurrence and 32 had distant metastasis.The positive rate of EBV-DNA in patients with metastasis was significantly higher than that in patients without recurrence and metastasis(P<0.05),T-cadherin mRNA in patients without recurrence and metastasis was higher than that in patients with recurrence and metastasis(P<0.05).PCNA mRNA in patients without recurrence and metastasis was lower than that in pa-tients with r

关 键 词：鼻咽肿瘤/病理学 钙结合蛋白质类 

分 类 号：R739.6[医药卫生—肿瘤]