青楷槭皮热水提取物的抗氧化性和抗菌性研究  

Antioxidant and antibacterial activity of hot water extract from Acer tegmentosum Maxim bark

作　　者：张鑫[1] 孙方丹 宋雨阳 金铁岩[1] ZHANG Xin;SUN Fangdan;SONG Yuyang;JIN Tieyan(College of Agriculture,Yanbian University,Yanji 133002,China)

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《延边大学学报（自然科学版）》2020年第1期61-64,共4页Journal of Yanbian University（Natural Science Edition）

摘　　要：为研究青楷槭皮热水提取物的抗氧化性和抗菌性,利用1,1二苯基2三硝基苯肼(DPPH)、2,2’联氮双(3乙基苯并噻唑啉6磺酸)二铵盐(ABTS)和铁离子的还原能力评价了青楷槭皮热水提取物的抗氧化性,并选用变形链球菌(3289)、金黄色葡萄球菌(4220)和大肠杆菌(1924)对热水提取物的抗菌性进行了研究.结果表明:青楷槭皮热水提取物具有良好的抗氧化性,且抗氧化性与提取物浓度呈明显量效关系.在测定浓度范围内,青楷槭皮热水提取物对DPPH的清除率可达95.56%,对ABTS的清除率可达94.76%,对铁离子还原力吸光值可达2.60;青楷槭皮热水提取物的乙酸乙酯和水层仅对大肠杆菌(1924)具有抗菌性,最小抑菌浓度(MIC)分别为128μg/mL和16μg/mL.以上实验结果可为开发利用青楷槭资源提供参考.In order to discuss the antioxidant and antibacterial activity of hot water extract from Acer tegmentosum Maxim bark,the 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS),and Ferric-reducing power is used to evaluate the antioxidant activity of hot water extract from Acer tegmentosum Maxim bark,and Streptococcus mutans(3289),Staphylococcus aureus(4220)and Escherichia coli(1924)is used to investigate the antibacterial activity of hot water extract.The results show that the hot water extract exhibit evident antioxidant effect,in addition there was a significant dose effect relationship between antioxidant activity and extract concentration.Within the range of measured concentration,DPPH and ABTS scavenging rate,Ferric-reducing power of hot water extract reach 95.56%,94.76%and 2.60,respectively.The ethyl acetate and water layer of hot water extract have antibacterial activity only to E.coli(1924),further the minimum inhibitory concentration(MIC)is 128μg/mL,16μg/mL,respectively.The above experimental results can provide references for the development and utilization of Acer tegmentosum Maxim resources.

关 键 词：青楷槭皮 热水提取物 抗氧化性 抗菌性 

分 类 号：TQ28[化学工程—有机化工]