低频重复经颅磁刺激对甲基苯丙胺成瘾大鼠复吸行为、记忆功能和海马结构的影响  

Effects of low-frequency repetitive transcranial magnetic stimulation on relapse behavior, memory function and hippocampus in rats with methamphetamine addiction

作　　者：徐晓津[1] 房茂胜[1] 缪楹 徐艳芩 Xu Xiaojin;Fang Maosheng;Miao Ying(Dept of Psychiatry,Wuhan Mental Health Center,Wuhan 430012)

机构地区：[1]武汉市精神卫生中心精神科,武汉430012

出　　处：《安徽医科大学学报》2020年第4期503-508,共6页Acta Universitatis Medicinalis Anhui

基　　金：湖北省卫生和计划生育委员会联合基金项目(编号:WJ2018H1559);武汉市卫生和计划生育委员会科研项目(编号:WX18D32)。

摘　　要：目的研究低频重复经颅磁刺激(rTMS)对甲基苯丙胺(MA)成瘾大鼠复吸行为、记忆功能和海马结构的影响。方法将60只SD大鼠随机分为对照组、模型组、实验组,将模型组和实验组大鼠利用固定比例程序建立MA大鼠自身给药模型,在模型建立后至自然戒断成功过程中,对实验组大鼠进行rTMS治疗7 d,模型组和对照组仅进行假刺激处理,完成后对各组大鼠进行旷野实验和Morris水迷宫实验判断其学习记忆能力;对模型组和实验组大鼠给予条件线索诱导后,观察其是否出现复吸行为;实验结束后处死大鼠取大脑海马组织进行切片,经HE染色、尼氏染色及醋酸双氧铀-枸橼酸铅染色后观察各组海马组织结构变化情况。结果成功制备MA成瘾大鼠模型,并且模型组大鼠的学习记忆功能降低,rTMS治疗后可有效改善MA成瘾大鼠的学习记忆功能降低情况;模型组大鼠自然戒断后给予条件线索会诱导出现复吸行为,而经rTMS治疗后,在条件线索诱导下其有效鼻触碰次数明显减少;MA成瘾大鼠模型海马组织中尼氏小体数目及平均光密度均降低,CA3区神经突触数量减少,前、后膜有融合情况,突触小体数量减少,rTMS治疗后可以一定程度上缓解这种病理改变。结论 rTMS治疗能够改善MA成瘾大鼠的记忆功能及海马神经元损伤,还能抑制条件线索诱导下MA复吸行为。Objective To study effects of low-frequency repetitive transcranial magnetic stimulation(rTMS) on relapse behavior, memory function and hippocampus of rats with methamphetamine(MA) addiction.Method 60 SD rats were randomly divided into control group, model group and experimental group. Fixed ratio 1 was applied in model group and experimental group to establish self-administration models of MA rats. In the process from model establishment to successful natural withdrawal, rats in experimental group were treated with rTMS for 7 d, while rats in model group and control group were only treated with sham stimulation. After completion, learning and memory ability of rats in each group was determined by wilderness experiment and Morris water maze test. After rats in model group and experimental group were given conditional clue induction, presence or absence of relapse behaviors was observed. After the end of the experiment, rates were sacrificed. The cerebral hippocampus was taken for slicing up. After HE staining,Nissl staining and uranyl acetate-lead citrate staining,changes of hippocampus were observed. Results The MA addiction rat models were successfully prepared. The learning and memory ability in model group was reduced. The r TMS treatment could effectively improve learning and memory treatment of rats with MA addiction. After natural withdrawal,there were relapse behaviors in rats of model group given conditional clue induction. After r TMS treatment,number of effective nasal touching under conditional clue induction was significantly decreased. In hippocampus of MA addiction rat models,number of Nissl bodies and average optical density decreased. The number of synapses in CA3 area reduced. There was fusion in anterior and posterior membranes.The number of synaptosomes reduced. The r TMS treatment could alleviate pathological changes to some extent.Conclusion The r TMS treatment can improve memory function and hippocampus tissue damage in MA addiction rats,and inhibit MA relapse behavior under conditional clu

关 键 词：甲基苯丙胺 成瘾 低频重复经颅磁刺激 复吸 

分 类 号：R749.92[医药卫生—神经病学与精神病学] R745[医药卫生—临床医学]