循环肿瘤细胞与其多巴胺受体1表达联合检测在乳腺癌疗效监控中的应用研究  

The application research of CTCs and its DRD1 expression combined detection on curative effect monitoring of breast cancer

作　　者：徐彬[1] 杨清峰 赵小玉 余元勋[1] 李建平[1] 刘萍[1] 冷雪峰 王迎新[1] Xu Bin;Yang Qingfeng;Zhao Xiaoyu(AnHui Medical College,AnHui Prouiner Medical Genetics Center,Hefei 230061;Anhti Women and Child Health Care Hospital,Hefei 230001;Shanghai Baoteng Medical Laboratory Co.,Ltd,Shanghai 201204)

机构地区：[1]安徽医学高等专科学校,安徽省优生优育医学遗传中心,合肥230061 [2]安徽省妇幼保健院,合肥230001 [3]上海宝藤医学检验所有限公司,上海201204

出　　处：《安徽医科大学学报》2020年第4期597-601,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校省级自然科学研究项目(编号:KJ2018A0798)。

摘　　要：目的研究乳腺癌患者外周血中的循环肿瘤细胞(CTCs)和其多巴胺受体1(DRD1)的表达水平对乳腺癌进展、疗效监控的影响。方法对20例健康对照者和20例未治疗的乳腺癌患者,采集其外周血,通过差相富集-免疫荧光原位杂交技术(SE-iFISH)及荧光定量PCR技术(Q-PCR),检测CTCs、CTCs DRD1的表达水平。之后在20例患者中,选择6例进行手术的乳腺癌患者,并在术后3、6个月,采集外周血,检测CTCs和CTCs DRD1的表达水平,并分析其与疾病进展的关系。结果20例健康对照者外周血中均未检测出CTCs;20例未治疗的乳腺癌患者检出CTCs 18例(90.0%),有较高的灵敏度和特异度。3例T2期乳腺癌患者术后3、6个月时均未出现复发和转移,CTCs和CTC DRD1的水平呈现下降趋势。3例T3期乳腺癌患者术后3、6个月时出现了复发和转移,CTCs和CTCs DRD1的水平呈现升高趋势。结论在CTCs高检出率和CTCs DRD1高表达的乳腺癌患者,随访常会提示预后不良;相反,在CTCs低检出率和CTCs DRD1低表达的患者中,随访提示疾病控制佳、预后较好。因此,无创的CTCs和CTCs DRD1检测,在乳腺癌疗效监控中,可能有较高的临床应用价值。Objective To study the effect of the circulating tumor cells(CTCs) and the CTCs dopamine receptor 1( DRD1) expression level in peripheral blood of breast cancer patients on breast cancer progression and curative effect monitoring. Methods Peripheral blood was collected from 20 healthy people and 20 untreated breast cancer patients, and CTCs and CTCs DRD1 expression levels of them were detected through the differential phase concentration-immune fluorescence in situ hybridization technique(SE-iFISH) in combination with the fluorescent quantitative PCR(Q-PCR). Then 6 cases of breast cancer were selected for operation, and their peripheral blood was collected at 3 and 6 months after operation, to detect CTCs and CTCs DRD1 expression levels and analyze their relationship with disease progression and curative effect. Results CTCs were not detected in peripheral blood of 20 healthy persons while detected in 18 of 20(90.0%) untreated breast cancer patients, which presented high sensitivity and specificity. CTCs and CTCs DRD1 levels evidently decreased in 3 T2 stage patients 3 months and 6 months after surgery(they did not appear recurrence and metastasis). On the contrary, CTCs and CTCs DRD1 levels evidently increased in 3 T3 stage patients 3 months and 6 months after surgery(they appeared prominent recurrence and metastasis). Conclusion Breast cancer patients with high CTCs detection rate and CTCs DRD1 expression often have poor prognosis. On the contrary, patients with low CTCs detection rate of CTCs and expression of CTCs DRD1 show better disease control and good prognosis. Hence, noninvasive CTCs and CTCs DRD1 detection may have high clinical application value in monitoring the efficacy of breast cancer.

关 键 词：循环肿瘤细胞 多巴胺受体1 乳腺癌 疗效评估 

分 类 号：R733[医药卫生—肿瘤]