lncRNA FEZF1-AS1在胶质瘤中的表达水平及其生物学功能  

Expression level and biological function of lncRNA FEZF1-AS1 in glioma

作　　者：赵玉红[1] 高阳 赵洪卫 李勐[2] 邵伟[2] Zhao Yuhong;Gao Yang;Zhao Hongwei(Dept of Disease Science,The Affiliated Hospital of Binzhou Medical University,Binzhou 256603;Dept of Neurosurgery,The Affiliated Hospital of Binzhou Medical University,Binzhou 256603)

机构地区：[1]滨州医学院附属医院病理科,滨州256603 [2]滨州医学院附属医院神经外科,滨州256603

出　　处：《安徽医科大学学报》2020年第5期767-771,共5页Acta Universitatis Medicinalis Anhui

基　　金：山东省高等学校科技计划项目(编号:J13LL07)。

摘　　要：目的研究长链非编码RNA FEZ家族锌指1反义RNA 1(lncRNA FEZF1-AS1)在胶质瘤组织中的表达及其临床意义,并探讨FEZF1-AS1在胶质瘤中发挥的生物学功能。方法qRT-PCR检测FEZF1-AS1在40例胶质瘤组织和40例正常脑组织中的表达,并分析胶质瘤组织中FEZF1-AS1的表达与患者临床病理参数的关系;Kaplan-Meier生存曲线分析FEZF1-AS1表达对患者预后的影响。FEZF1-AS1敲减质粒转染胶质瘤细胞系U87抑制FEZF1-AS1的表达后,MTS实验检测细胞的增殖能力;Boyden实验检测细胞的侵袭能力;TOP/FOP报告基因检测Wnt/β-catenin信号通路;Western blot检测细胞中β-catenin蛋白的表达。结果qRT-PCR结果显示FEZF1-AS1在胶质瘤组织中的表达高于正常脑组织,其高表达与WHO临床分级相关,FEZF1-AS1表达高的胶质瘤患者生存期较短。干扰U87细胞中FEZF1-AS1的表达后,细胞增殖和侵袭能力均下降,TOP/FOP报告基因荧光素酶活性降低,细胞中β-catenin的蛋白表达减少。结论FEZF1-AS1在胶质瘤组织中表达上调,且其表达与WHO临床分级及不良预后相关。干扰胶质瘤细胞中FEZF1-AS1的表达可能通过调控Wnt/β-catenin信号通路抑制细胞增殖和侵袭能力。Objective To study the expression and clinical significance of long non-coding RNA FEZ family(lncRNA FEZF1-AS1)in glioma tissues,and to explore the biological function of FEZF1-AS1 in gliomas.Methods qRT-PCR was used to detect the expression of FEZF1-AS1 in 60 glioma tissues and 40 normal brain tissues,and the relationship between the expression of FEZF1-AS1 in glioma tissues and clinical pathological parameters was analyzed.Kaplan-Meier survival curve analyzed the effect of FEZF1-AS1 expression on patient prognosis.FEZF1-AS1 shRNA transfected glioma cell line U87 inhibited the expression of FEZF1-AS1,MTS assay was used to detect cell proliferation.Boyden assay was used to detect cell invasion.TOP/FOP reporter gene detection was used to detect Wnt/β-catenin signaling pathway.Western blot was used to detect the expression ofβ-catenin protein in cells.Results The expression of FEZF1-AS1 in glioma tissues was higher than that in normal brain tissues.The high expression of FEZF1-AS1 was associated with WHO clinical classification.The glioma patients with high FEZF1-AS1 expression had shorter survival time.After interfering with the expression of FEZF1-AS1 in U87 cells,the proliferation and invasion ability of cells decreased,the luciferase activity of TOP/FOP reporter gene decreased,and the protein expression ofβ-catenin decreased.Conclusion The expression of FEZF1-AS1 is up-regulated in glioma tissues,and its expression level is related to WHO clinical grade and poor prognosis.Interfering with the expression of FEZF1-AS1 in glioma cells may inhibit cell proliferation and invasion by regulating Wnt/β-catenin signaling pathway.

关 键 词：胶质瘤 lncRNA FEZF1-AS1 增殖 侵袭 WNT/Β-CATENIN 

分 类 号：R739.41[医药卫生—肿瘤]