肝细胞癌中膜铁转运蛋白的表达及意义  

The expression and significance of FPN in hepatocellular carcinoma

作　　者：王珊珊 汪静 张浩[1] 王琴[1] 杨凯[1] 管世鹤[1] Wang Shanshan;Wang Jing;Zhang Hao(Dept of Clinical Laboratory,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院检验科,合肥230601

出　　处：《安徽医科大学学报》2020年第5期772-775,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省科技厅重点研究与开发计划项目(编号:1804h08020236);安徽省转化医学研究院科研基金项目(编号:2017zhyx11)。

摘　　要：目的探讨膜铁转运蛋白(FPN)在肝细胞癌中的表达及其意义。方法采用ELISA法分别检测41例健康对照组、48例慢性肝病组和80例肝癌组血清中的FPN表达,分析肝癌组FPN表达程度与肝癌患者Child-pugh分级,巴塞罗那(BCLC)肝癌分期等指标的相关性;收集46例肝组织切片,通过普鲁士蓝铁染色和免疫组化检测肝良性病变组织和肝癌组肝组织铁沉积及FPN表达。结果血清中健康对照组与慢性肝病组FPN表达差异无统计学意义(P>0.05),而肝癌组FPN表达明显高于健康对照组(P<0.001)和慢性肝病组(P<0.05),且FPN表达程度与肝癌的Child-pugh分级,BCLC肝癌分期及转移呈正相关(P<0.05),与肿瘤大小(P>0.05),结节数目(P>0.05)不相关;组织中,肝癌患者体内出现蓝色铁颗粒沉积现象且肝癌组FPN的表达明显高于肝良性病变组(P<0.05)。结论FPN在肝细胞癌患者体内中高表达,表明FPN可能在肝细胞癌的发生发展中起到重要作用,可作为肝细胞癌免疫治疗的一个新的靶点。Objective To investigate the expression and significance of ferroportin(FPN)in hepatocellular carcinoma.Methods The expression of FPN in serum of 41 healthy controls,48 chronic liver disease patients and 80 hepatocellular carcinoma patients was detected by ELISA,and the correlation between the degree of FPN in hepatocellular carcinoma group and Child-pugh classification,Barcelona(BCLC)staging of hepatocellular carcinoma was analyzed.46 cases of liver tissue were collected,and the iron deposition and FPN expression of liver tissue were detected by Prussian blue iron staining and immunohistochemistry.Results In serum,there was no significant difference between healthy control group and chronic liver disease group(P>0.05),but the expression of FPN in hepatocellular carcinoma group was significantly higher than that in healthy control group(P<0.001)and chronic liver disease group(P<0.05).The degree of FPN was positively correlated with the grade of Child-pugh,staging of BCLC(P<0.05)and metastasis,but not with tumor size(P>0.05)and number of nodules(P>0.05).The deposition of blue iron particles was found in patients with hepatocellular carcinoma,and the expression of FPN in liver cancer tissue group was significantly higher than that in benign liver disease tissue group(P<0.05).Conclusion FPN is highly expressed in patients with hepatocellular carcinoma,indicating that FPN may play an important role in the occurrence and development of hepatocellular carcinoma,and it can be used as a new target for immunotherapy of hepatocellular carcinoma.

关 键 词：肝细胞癌 膜铁转运蛋白 铁死亡 

分 类 号：R735.7[医药卫生—肿瘤]