蛋白酶体β5过表达对人晶状体上皮细胞抗氧化能力的影响  

The Effect of Overexpression of Proteasome PSMB5 on Antioxidative Ability of Human Lens Epithelial Cells

作　　者：张铁英 袁钊辉[2] 娄秉盛[2] Tieying Zhang;Zhaohui Yuan;Bingsheng Lou(Department of Ophthalmology,Affiliated Hexian Memorial Hospital,Southern Medical University,Hexian Memorial Medical Hospital of Panyu District,Guangzhou 511400,China;Zhongshan Ophthalmic Center,Sun Yat-Sen University,State Key Laboratory of Ophthalomology,Guangzhou 510060,China)

机构地区：[1]南方医科大学附属何贤纪念医院/广州市番禺区何贤纪念医院眼科,511400 [2]中山大学中山眼科中心/中山大学眼科学国家重点实验室,广州510060

出　　处：《中华眼视光学与视觉科学杂志》2020年第4期305-309,共5页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：广州市医药卫生科技项目(20171A010336)。

摘　　要：目的:研究蛋白酶体β5(PSMB5)过表达对人晶状体上皮细胞抗氧化能力的影响。方法:实验研究。构建重组质粒pcDNA3.1-PSMB5,将其转染入人晶状体上皮细胞株SRA01/04中,形成稳定表达(作为实验组),设pcDNA3.1空载体作为对照组。将2组细胞置于40 μmol/L H 2O 2环境下,Hoechst 33342荧光染色观察2组细胞核形态的变化;流式细胞仪检测2组细胞凋亡率的变化。数据采用独立样本 t检验。 结果:40 μmol/L H 2O 2环境下,Hoechst 33342荧光染色可见,与实验组相比,对照组的细胞核明显皱缩、变形;流式细胞仪检测发现,实验组和对照组的细胞凋亡指数分别为(9.3±0.9)%和(15.7±1.9)%,均高于处理前的(5.9±0.8)%和(7.1±1.1)%,对照组凋亡指数比实验组的凋亡指数更高( t=3.742, P=0.008)。 结论:PSMB5基因过表达能有效提高人晶状体上皮细胞的抗氧化能力。Objective:To discuss the antioxidative ability of proteasome subunitβ5(PSMB5)over expression in human lens epithelial cells.Methods:This is an experimental study.Recombinant plasmid pcDNA3.1-PSMB5 was constructed and transfected into a human lens epithelium strain SRA01/04 to form a stable transfection(experimental group),and empty pcDNA3.1 was also transfected into lens epithelial cells at the same time as a control.Two groups of cells were treated with 40μmol/L H2O2.The nuclear morphology of the two group cells was observed by Hoechst 33342 stain,and cell apoptosis of the two group cells was dectected by flow cytometry.Data was analyzed by a paired t test.Results:Under a 40μmol/L H2O2 treated environment,Staining by Hoechst 33342 indicated that the nuclei in the control group were significantly shrunken and condensed compared with those of the experimental group.The apoptotic percentages detected by flow cytometry in the experimental group and control group were(9.3±0.9)%and(15.7±1.9)%,and both had increased compared to pre-treatment with H2O2,which were(5.9±0.8)%and(7.1±1.1)%,respectively.The apoptotic percentage in the control group increased to a higher level than in the experimental group(t=3.742,P=0.008).Conclusions:Overexpression of proteasome PSMB5 can effectively improve the antioxidative ability of human lens epithelial cells.

关 键 词：过表达 PSMB5 蛋白酶体 晶状体 抗氧化 

分 类 号：R776.1[医药卫生—眼科]