代谢酶基因多态性与结直肠癌临床病理特征的关系  

Relationship between metabolic enzyme gene polymorphism and clinical progression of colorectal cancer

作　　者：孔晓玲 张晓梅[2] 洪伟伟 张琰[2] 沈波[2] 程艳萍 杨圣 梁戈玉[1] Xiao-ling Kong;Xiao-mei Zhang;Wei-wei Hong;Yan Zhang;Bo Shen;Yan-ping Cheng;Sheng Yang;Ge-yu Liang(School of Public Health,Southeast University,Nanjing,Jiangsu 210009,China;Jiangsu Cancer Hospital,Nanjing,Jiangsu 210009,China)

机构地区：[1]东南大学公共卫生学院,江苏南京210009 [2]江苏省肿瘤医院,江苏南京210009

出　　处：《中国现代医学杂志》2020年第9期20-27,共8页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:81673132)。

摘　　要：目的探讨代谢酶基因谷胱甘肽S-转移酶M3(GSTM3)、二氢嘧啶脱氢酶基因(DPYD)、UDP葡糖醛酸基转移酶1家族多肽A1(UGT1A1)单核苷酸多态性(SNP)与结直肠癌临床病理特征的关系。方法选取2016年1月—2017年7月江苏省肿瘤医院收集的结直肠癌患者外周血690份,使用TaqMan实时荧光定量PCR法对GSTM3 rs7483、DPYD rs1801159、UGT1A1 rs37553193个位点进行基因分型,应用Logistic回归分析3个多态性位点与结直肠癌临床特征的关系。结果隐性模型中GSTM3 rs7483 GG基因型在Ⅰ、Ⅱ期的频率高于Ⅲ、Ⅳ期(P<0.05),分层分析发现,在>60岁患者中,隐性模型中GG基因型携带者Ⅰ、Ⅱ期肿瘤比例是AA+GA基因型的0.321倍(P<0.05)。DPYD rs1801159 AG+GG基因型携带者低分化肿瘤比例是AA基因型的1.398倍(P<0.05),尤其与低龄(≤60岁)和女性患者的低分化有关系(P<0.05)。未发现UGT1A1 rs3755319与结直肠癌临床病理特征的关系(P>0.05)。结论代谢酶基因GSTM3 rs7483、DPYD rs1801159多态性可能影响结直肠癌的临床进展。Objective To investigate the relationship between the metabolic enzyme genes GSTM3,DPYD,UGT1A1,single nucleotide polymorphisms(SNP)and the clinical progression of colorectal cancer.Methods Totally 690 peripheral blood samples were collected from patients with colorectal cancer from January 2016 to July 2017.TaqMan real-time fluorescence quantitative PCR was used for genotyping of three sites of GSTM3 rs7483,DPYD rs1801159 and UGT1A1 rs3755319.Logistic regression was used to analyze the relationship between three polymorphic sites and colorectal cancer.Results The frequency of GSTM3 rs7483 GG genotype was significantly higher in stage I and II than in stage III and IV(P<0.05).Hierarchical analysis found that in elderly(>60 years old)patients,the GG carriers in the recessive model were 0.321 times higher than AA+GA genotypes(P<0.05).The poorly differentiated tumors of DPYD rs1801159 AG+GG genotype were 1.398 times of AA genotype(P<0.05),especially the poor differentiation of patients with low age≤60 years and female patients(P<0.05).No relationship between UGT1A1 rs3755319 and clinical progression of colorectal cancer was found(P>0.05).Conclusion Metabolic enzyme genes GSTM3 rs7483 and DPYD rs1801159 may affect the clinical progression of colorectal cancer.

关 键 词：结直肠肿瘤 代谢酶基因 单核苷酸多态性 临床进展 分层分析 

分 类 号：R735[医药卫生—肿瘤]