Wnt-1诱导分泌蛋白2对胃癌细胞上皮间质转化的影响  

Effect of WISP2 on epithelial-mesenchymal transformation in gastric cancer cells

作　　者：任春梅[1] 许斌[2] 邹文蓉[3] 彭鹏 文黎明[1] Chun-mei Ren;Bin Xu;Wen-rong Zou;Peng Peng;li-ming Wen(Department of Gastroenterology,Sichuan Mianyang 404 Hospital,Mianyang,Sichuan 621000,China;Department of infection,Sichuan Mianyang 404 Hospital,Mianyang,Sichuan 621000,China;Department of Hematology and Oncology,Sichuan Mianyang 404 Hospital,Mianyang,Sichuan 621000,China)

机构地区：[1]四川绵阳四〇四医院消化内科,四川绵阳621000 [2]四川绵阳四〇四医院感染科,四川绵阳621000 [3]四川绵阳四〇四医院血液肿瘤科,四川绵阳621000

出　　处：《中国现代医学杂志》2020年第9期38-42,共5页China Journal of Modern Medicine

基　　金：四川省卫生厅科学研究项目(No:16PJ376)。

摘　　要：目的探讨Wnt-1诱导分泌蛋白2(WISP2)在胃癌组织中的表达及其对胃癌患者癌细胞上皮间质转化的影响。方法选取2016年3月—2018年5月四川绵阳四〇四医院收治的胃癌患者癌组织和癌旁组织标本,每组85例。采用免疫组织化学法检测WISP2在胃癌组织和癌旁组织中的表达情况;构建WISP2-shRNA和NC-shRNA稳定细胞系,采用细胞划痕实验检测WISP2下调对胃癌细胞迁移的影响;采用Transwell实验检测WISP2下调对胃癌细胞侵袭的影响;采用Western blotting检测WISP2下调对胃癌细胞E-钙黏蛋白(E-cadherin)、神经-钙黏蛋白(N-cadherin)和波形蛋白(Vimentin)表达的影响。结果WISP2在胃癌组织(61.2%)中的阳性表达率高于癌旁组织(11.8%)(P<0.05);与NC-shRNA组(84.74±9.61)%比较,WISP2-shRNA组(57.18±6.05)%细胞划痕愈合程度下降(P<0.05);WISP2-shRNA组细胞侵袭个数(195.36±23.45)较NC-shRNA组(404.91±38.16)减少(P<0.05);与NC-shRNA组比较,WISP2-shRNA组细胞E-Cadherin的表达水平升高(P<0.05),N-Cadherin和Vimentin的表达水平下降(P<0.05)。结论W ISP2在胃癌组织中高表达,下调WISP2的表达可抑制胃癌细胞的上皮间质转化和侵袭转移。Objective To investigate the expression of Wnt-inducible secreted protein 2(WISP2)in gastric cancer and its effect on epithelial-mesenchymal transition.Methods A total of 85 specimens of tumor tissues and adjacent tissues of gastric cancer patients from March 2016 to May 2018 were selected.The WISP2 expression in gastric cancer tissues and adjacent tissues was detected by immunohistochemistry.The WISP2-shRNA and NCshRNA stable cell lines were constructed,and the effect of WISP2 down-regulation on migration of gastric cancer cells was analyzed by scratch wound healing assay.Transwell was used to detected the effect of WISP2 downregulation on the invasion of gastric cancer cells.The effects of WISP2 down-regulation on the expression of E-cadherin,N-cadherin and Vimentin in gastric cancer cells were detected by western blotting.Results The positive expression rate of WISP2 in gastric cancer tissues(61.2%)was significantly higher than that in adjacent tissues(11.8%)(P<0.05).Compared with the NC-shRNA group(84.74±9.61)%,the degree of cell healing in WISP2-shRNA group was significantly decreased(57.18±6.05)%(P<0.05).The number of cell invasion in the WISP2-shRNA group(195.36±23.45)was significantly lower than that in the NC-shRNA group(404.91±38.16)(P<0.05).Compared with NC-shRNA group,the expression of E-cadherin was significantly increased in WISP2-shRNA group,while the expression of N-Cadherin and Vimentin was significantly down-regulated(P<0.05).Conclusion WISP2 is highly expressed in gastric cancer tissues,and down-regulation of WISP2 expression can significantly inhibit EMT,invasion and metastasis of gastric cancer cells.

关 键 词：胃肿瘤 WISP2 上皮间质转化 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]