Comparative Transcriptome Analysis Reveals Different Host Cell Responses to Acute and Persistent Foot-and-Mouth Disease Virus Infection  

作　　者：Jiadai Li Lingling Han Yao Hao Yuncong Yuan Mingzhen Wang Xiu Xin Hailong Wang Fang Yu Congyi Zheng Chao Shen 

机构地区：[1]State Key Laboratory of Virology,College of Life Sciences,Wuhan University,Wuhan 430072,China [2]Department of Pathology,Zhongnan Hospital,Wuhan University,Wuhan 430072,China [3]China Center for Type Culture Collection,Wuhan University,Wuhan 430072,China

出　　处：《Virologica Sinica》2020年第1期52-63,共12页中国病毒学（英文版）

基　　金：the financial support of the National Infrastructure of Natural Resources for Science and Technology Program(No.2011-572);the National Natural Sciences Foundation of China(No.31370185);the National Basic Research Program of China(No.2011CB504800)to Prof.Congyi Zheng;the Fundamental Research Funds for the Central Universities(2042018kf0241);the National Science and Technology Infrastructure Grants(NSTI-CR15 and NSTI-CR16)to Dr.Chao Shen.

摘　　要：Foot-and-mouth disease virus(FMDV) rapidly causes cytopathic effects in susceptible cells. Incomplete viral clearance during the acute infection leads to persistent infection. The relationship between host gene expression and the persistent infection remains unclear. In this study, we analyzed the transcriptome profiles of BHK-21 cells acutely and persistently infected with FMDV to identify differences in gene expression. GO and KEGG enrichment analyses showed that the 8,378 differentially expressed genes were significantly enriched in categories including metabolism, biosynthesis, ribosome function, and endocytosis. In persistently infected BHK-21 cells, ribosome-and translation-related genes were significantly down-regulated. There were more differentially expressed immune-related genes during persistent infection than during acute infection. Two hundred and seventy-four genes were differentially expressed in both acutely and persistently infected BHK-21 cells. Among these genes, heat shock protein family B member 1(Hspb1) knockdown significantly inhibited FMDV replication. Our research provides a basis for further research to understand the mechanisms of persistent FMDV infection including the genes involved in FMDV replication.

关 键 词：Foot-and-mouth disease virus(FMDV) PERSISTENT infection RNA-Seq DIFFERENTIALLY EXPRESSED gene(DEG) 

分 类 号：S852.65[农业科学—基础兽医学]