miRNA-lncRNA相互作用影响人NK细胞与树突状细胞免疫功能和胃癌细胞侵袭力的分子机制  

Molecular Mechanism of miRNA-lncRNA Interaction Affecting the Immune Function of Human NK Cells and Dendritic Cells and the Invasiveness of Gastric Cancer Cells

作　　者：王保全 王战红 王旭 WANG Baoquan;WANG Zhanhong;WANG Xu(Department of General Surgery, Weinan Central Hospital, Shannxi Weinan 714000, China)

机构地区：[1]渭南市中心医院普外科,陕西渭南714000

出　　处：《中国医药导刊》2020年第4期265-269,共5页Chinese Journal of Medicinal Guide

摘　　要：目的:研究miRNA-lncRNA相互作用影响人自然杀伤细胞(NK)和树突状细胞(DC)免疫功能和胃癌(GC)细胞侵袭力的分子机制。方法:实验分为4组:GC组织、正常组织、GC细胞系、正常细胞系,通过定量实时聚合酶链反应(qRT-PCR)检测lncRNA(MEG3)在4个实验组的表达;通过CCK-8检测NK细胞与DC细胞的活力;通过Transwell测定法测量GC细胞迁移和侵袭能力;通过荧光素酶活性检测miRNA(miR-21)和MEG3之间的关系;通过蛋白质免疫检测miR-21模拟物转染或MEG3转染后,NK细胞免疫功能标记蛋白(CD56)、DC细胞免疫功能标记蛋白(CD80)和细胞侵袭标记蛋白(MMP-2和MMP-9)的表达量。结果:MEG3在GC组织和细胞中下调表达(P<0.05);MEG3的过表达抑制了GC的NK细胞与DC细胞的活力以及GC细胞迁移和侵袭能力(P<0.05);过表达的miR-21导致MEG3-WT荧光素酶活性降低,但对MEG3-mut报告基因的荧光素酶活性没有明显影响;过表达的MEG3减弱了miR-21模拟物对CD56、CD80、MMP-2和MMP-9蛋白的表达(P<0.05)。结论:miRNA-lncRNA相互作用在人NK细胞与DC细胞免疫功能和GC细胞侵袭进展中具调节作用,其诱发MEG3的过表达,从而抑制了GC的NK细胞与DC细胞的活力。Objective:To study the molecular mechanism of miRNA-lncRNA interaction affecting the immune function of human NK cells and dendritic cells(DC)and the invasiveness of gastric cancer(GC)cells.Methods:The experiment was divided into 4 groups:GC tissue,normal tissue,GC cell line,normal cell line.lncRNA(MEG3)was detected by qRT-PCR in 4 experimental groups.NK cell and DC cell viability were detected by CCK-8.Migration and invasion of GC cells were measured by Transwell assay.Relationship between miRNA(miR-21)and MEG3 were detected by luciferase activity.After miR-21 mimic transfection or MEG3 transfection,the expression levels of NK cell immune function marker protein(CD56),DC cell immunological function marker protein(CD80)and cell invasion marker proteins(MMP-2 and MMP-9)were detected by protein immunoassay.Results:We found that MEG3 is down-regulated expressed in GC tissues and cells(P<0.05).Overexpression of MEG3 significantly inhibited NK cell and DC cell viability and the migration and invasion of GC cells(P<0.05).Overexpression of miR-21 resulted in MEG3-WT luciferase activity significantly decreased,but had no significant effect on the luciferase activity of the MEG3-mut reporter gene.Overexpression of MEG3 attenuated the expression of CD56,CD80,MMP-2 and MMP-9 proteins by miR-21 mimics(P<0.05).Conclusion:Study reveals that miRNA-lncRNA interactions regulate the immune function of human NK cells and DC cells and the progression GC cell invasion.It induces the overexpression of MEG3 and inhibites NK cell and DC cell viability.

关 键 词：MIRNA lncRNA NK细胞 树突状细胞 胃癌细胞 

分 类 号：R735.2[医药卫生—肿瘤]