褐球固氮菌在全红苋菜和小白菜叶际的定殖及其促生长效应  

Colonization and growth-promoting effects of Azotobacter chroococcum in phyllosphere of Amaranthus tricolor L.and Brassica campestris L.

作　　者：王燚凡 杨俊卿 吕耀 徐智敏 李取生 WANG Yi-fan;YANG Jun-qing;LYU Yao;XU Zhi-min;LI Qu-sheng(School of Environment,Jinan University,Guangzhou 511443,China;Guangzhou Research Institute of Environmental Protection,Guangzhou 510620,China;College of Environmental Science of Engineering,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)

机构地区：[1]暨南大学环境学院,广东广州511443 [2]广州市环境保护科学研究院,广东广州510620 [3]仲恺农业工程学院环境科学与工程学院,广东广州510225

出　　处：《江苏农业学报》2020年第2期336-342,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(41673094)。

摘　　要：通过土培盆栽试验,研究了叶面接种褐球固氮菌(Ac63)在全红苋菜(Amaranthus tricolor L.)和小白菜(Brassica campestris L.)叶际的定殖与促生效果,评估了在贫/富氮土培养且不/喷施接种Ac63处理下作物地上部生物量、叶绿素a和b含量及叶片总氮含量的变化,设计了Ac63的nifD基因特异性引物并通过qPCR分析其在叶际的丰度。结果表明,接种Ac63明显促进了贫氮土培养的全红苋菜地上部生物量、叶绿素a、b含量及叶片总氮含量增加(P<0.05),但对富氮土培养的全红苋菜仅促进叶绿素a含量增加(P<0.05);接种Ac63对贫/富氮土培养的小白菜均无明显作用(P>0.05)。qPCR结果显示Ac63能定殖于全红苋菜叶际而不能定殖于小白菜叶际;此外土壤速效氮可能通过增加叶片氮含量在一定程度上抑制了Ac63在全红苋菜的定殖数量及促生作用。说明固氮菌Ac63的叶际定殖具有作物选择性,对于能够定殖的作物具有良好固氮效果。The colonization of nitrogen-fixing bacteria(Ac63)in the phyllosphere of Amaranthus tricolor L.and Brassica campestris L.and growth-promoting effects were studied.A pot experiment was carried out to evaluate the changes of aboveground biomass,chlorophyll a and b contents,and total nitrogen content in leaves under different treatments nitrogen-poor and nitrogen-rich soil culture with and without spraying Ac63.The nifD-specific primers of Ac63 were designed,and their abundance in leaves was analyzed by qPCR.Results showed that inoculation with Ac63 could significantly promote the aboveground biomass,chlorophyll a and b contents,and total nitrogen content of A.tricolor L.cultured in nitrogen-poor soil(P<0.05).However,it only increased the chlorophyll a content of A.tricolor L.cultured in nitrogen-rich soil(P<0.05).Inoculation with Ac63 had no significant effect on B.campestris L.cultured in nitrogen-poor and nitrogen-rich soil(P>0.05).The qPCR results revealed that Ac63 could colonize in the phyllosphere of A.tricolor L.but not in the phyllosphere of B.campestris L.In addition,the soil available nitrogen could inhibit the colonization and growth of Ac63 in A.tricolor L.by increasing the nitrogen content of leaves to some extent.It is indicated that the phyllosphere colonization of nitrogen-fixing bacteria Ac63 has crop selectivity,and it has a good nitrogen fixation effect on the crop that can be colonized.

关 键 词：褐球固氮菌 叶际 定殖 qPCR 

分 类 号：S182[农业科学—农业基础科学] X172[环境科学与工程—环境科学]