燃煤来源的过量氟对仔鼠牙胚中釉原蛋白基因表达的影响  

Effects of excessive fluoride from coal source on amelogenin gene in rat offspring tooth germ

作　　者：顾瑜 田源 白国辉 陈彬 易思健 孔宁静 GU Yu;TIAN Yuan;BAI Guohui;CHEN Bin;YI Sijian;KONG Ningjing(Zhuhai Campus of Zunyi Medical University,Zhuhai,Guangdong 519041,China;Key Laboratory of Oral Disease Research Feature of Higher Schools in Guizhou Province,Zunyi,Guizhou 563000,China;Zunyi Medical University,Zunyi,Guizhou 563000,China)

机构地区：[1]遵义医科大学珠海校区,广东珠海519041 [2]贵州省高等学校口腔疾病研究特色重点实验室,贵州遵义563000 [3]遵义医科大学,贵州遵义563000

出　　处：《现代医药卫生》2020年第10期1441-1443,共3页Journal of Modern Medicine & Health

基　　金：国家自然科学基金资助项目(81960198);贵州省科技厅科学技术基金项目(黔科合J字〔2009〕2204号);贵州省遵义市汇川区科技局科技计划项目(遵汇科合201629)。

摘　　要：目的研究燃煤来源的过量氟对大鼠子代牙胚组织中釉原蛋白(AMEL)基因表达的影响。方法2017年5月选取3周龄SD大鼠18只,雌雄比例为2∶1,模拟高氟空气环境,每天饲以含氟玉米饲料,建立氟斑牙大鼠模型,其所产仔鼠作为研究组(n=6)。对照组为在正常空气环境中予以普通饲料所喂养的雌雄比例为2∶1的18只SD大鼠所产仔鼠(n=6)。取出生第1、3、5、7天仔鼠牙胚组织,采用荧光定量聚合酶链反应技术检测AMEL基因在牙胚发育过程中的表达变化。结果2组仔鼠牙胚组织中AMEL基因表达在仔鼠出生第3天达最高峰,第5天迅速降至最低;研究组仔鼠牙胚组织中AMEL基因表达水平明显低于对照组;2组仔鼠出生第1、3天牙胚组织中AMEL基因表达水平比较,差异均有统计学意义(P<0.05)。结论燃煤来源的氟可能在牙胚发育阶段通过影响AMEL基因的表达而影响牙齿的生长、发育。Objective To study the effect of excessive fluoride from coal source on the expression of amelogenin(AMEL gene in the tooth germ of rat offspring.Methods Eighteen SD rats aged 3 weeks were selected in May 2017.The female-male proportion was 2∶1.The high-fluorine air environment was simulated.The fluoride-containing corn diet was fed every day to establish the rat model of dental fluorosis.Their newborn rats served as the study group(n=6).The control group was the newborn rats born(n=6)by SD rats fed with normal diet in the normal air environment,and the female-male proportion was 2∶1.The tooth germ tissue of the offspring on 1,3,5,7 dof birth was taken out,and the expression change of AMEL gene in the development process of tooth germ was detected by adopting the fluorescent quantitative PCR.Results The expression level of AMEL gene in the tooth germ tissue of the two groups reached the highest peak on 3 dafter birth,and rapidly decreased to the lowest on 5 d.The expression level of AMEL gene in the tooth germ tissue of the study group was significantly lower than that in the control group(P<0.05).The AMEL gene expression level in the tooth germ tissue on 1,3 dafter birth had statistical differences between the two groups(P<0.05).Conclusion Coal-derived fluorine may affect the growth and development of teeth by affecting the expression of AMEL gene during the tooth germ development stage.

关 键 词：燃煤型氟斑牙 空气污染 动物疾病模型 大鼠 牙胚 釉原蛋白质 

分 类 号：R781.2[医药卫生—口腔医学]