锌对嗜酸乳杆菌生长及菌体内元素含量影响研究  

Effect of Zinc on the Growth and Element Content of Lactobacillus Acidophilus

作　　者：赵倩倩 闫赖赖[1,2] 解清 刘雅琼[1,2] 杨思宇 郭辰[1] 王京宇 ZHAO Qian-qian;YAN Lai-lai;XIE Qing;LIU Ya-qiong;YANG Si-yu;GUO Chen;WANG Jing-yu(Department of Health Inspection,School of Public Health,Peking University,Beijing 100191,China;Life Elementomics Laboratory,Medical and Health Analysis Center,Peking University,Beijing 100191,China)

机构地区：[1]北京大学公共卫生学院卫生检验学系,北京100191 [2]北京大学医药卫生分析中心生命元素组学实验室,北京100191

出　　处：《光谱学与光谱分析》2020年第5期1489-1494,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30972434)资助。

摘　　要：采用电感耦合等离子体质谱仪(ICP-MS)研究培养液中锌元素浓度变化对嗜酸乳杆菌生长及菌体内其他元素吸收状况的影响;从菌体中无机元素再平衡角度探讨锌干预影响嗜酸乳杆菌生长及元素吸收的潜在机制。以嗜酸乳杆菌为实验细菌菌种,以元素锌为实验元素,观察培养液中锌浓度变化所导致的嗜酸乳杆菌生长速度的变化。共设置7个锌元素浓度组,每组6个平行样品,锌元素浓度范围为0~1200 ng·mL^-1,每组浓度间隔200 ng·mL^-1,其中0 ng·mL^-1组即为对照组。利用电感耦合等离子体质谱仪(ICP-MS)测定培养液和嗜酸乳杆菌中的锌(Zn)、铜(Cu)、铁(Fe)、锰(Mn)等43种元素的含量;用分光光度计测定嗜酸乳杆菌的OD值,监控其生长速度。利用SPSS20.0(Statistical Package for the Social Science 20.0)统计软件对获得的实验数据进行统计分析。培养液中Zn浓度变化会引发嗜酸乳杆菌生长的刺激效应,在Zn浓度为600 ng·mL^-1时刺激效应最明显,同时导致菌体内无机元素浓度发生改变,菌体内包括Se和Fe在内的部分有益元素含量随着细菌生长速度的加快而升高,Cd等有害元素的含量则降低。相关分析显示,菌体中Se,U,Fe,Al,Ti,Sb,Ba,Mn,Co,Zn和Sr(按照2~10 h整体相关系数值由高到低排列)11种元素的含量与OD值之间具有较好的相关性,其中U,Al,Ti和Sb为负相关,其余7种元素为正相关;嗜酸乳杆菌中Se,Sr,Al,Mn,Fe,Co和Cd等14种元素含量与菌体中锌元素含量相关有统计学意义(p<0.05)。培养液中锌元素浓度变化会刺激或抑制嗜酸乳杆菌生长速度,同时,还可上调某些有益元素及下调某些有害元素元素的含量;菌体中有益与有害元素含量的再平衡或许是培养液中锌干预改变嗜酸乳杆菌生长速度的潜在机制。To investigate the effects of zinc concentration on the growth of Lactobacillus acidophilus and other elements in the bacteria by inductively coupled plasma mass spectrometry(ICP-MS).The potential mechanism of growth and elemental absorption of Lactobacillus acidophilusafterzinc interventionwas investigatedon the re-equilibrium of inorganic elements in the cells.Lactobacillus acidophilus was used as the experimental bacterial strain,and elemental zinc was used as the experimental element to observe the change of growth rate of Lactobacillus acidophilus caused by the change of zinc concentration in the culture solution.A total of 7 zinc concentration groups were set up,and each group of 6 parallel samples had a zinc concentration ranging from 0 to 1200 ng·mL^-1.The concentration interval of each group was 200 ng·mL^-1,and the 0 ng·mL^-1 group was the control group.Determination of 43 elements such as zinc(Zn),copper(Cu),iron(Fe)and manganese(Mn)in culture medium and Lactobacillus acidophilus by inductively coupled plasma mass spectrometry(ICP-MS);The photometer measures the OD value of Lactobacillus acidophilus and monitors its growth rate.Statistical analysis was performed on the obtained experimental data using SPSS 20.0(Statistical Package for the Social Science 20.0)statistical software.The change of Zn concentration in the culture solution caused the stimulation effect of Lactobacillus acidophilus growth.The stimulating effect was most obvious when the concentration of Zn was 600 ng·mL^-1.At the same time,the concentration of inorganic elements in the bacteria changed.The bacteria included Se and Fe in the bacteria.The content of some beneficial elements in the interior increases with the increase of bacterial growth rate,and the content of harmful elements such as Cd decreases.Correlation analysis showed that the content of 11 elements in Se,U,Fe,Al,Ti,Sb,Ba,Mn,Co,Zn,Sr(arranged from high to low according to the overall correlation coefficient of 2~10 hours)There is a good correlation between OD values,i

关 键 词：锌(Zn) 嗜酸乳杆菌 刺激效应 电感耦合等离子体质谱仪 

分 类 号：R117[医药卫生—公共卫生与预防医学]