热休克蛋白A2、IGFBP-2在胰腺癌患者的表达及与临床病理特征相关性  

Expression of heat shock protein A2 and IGFBP-2 in patients with pancreatic cancer and its correlation with clinical pathological features

作　　者：于洋[1] 薛莹 仓顺东[1] YU Yang;XUE Ying;CANG Shundong(Department of oncology,henan provincial people's hospital,Zhengzhou,Henan,China,450003)

机构地区：[1]河南省人民医院肿瘤内科一病区,河南郑州450003

出　　处：《分子诊断与治疗杂志》2020年第3期371-375,共5页Journal of Molecular Diagnostics and Therapy

基　　金：河南省科技厅基础与前沿技术研究项目(152300410154)。

摘　　要：目的探究热休克蛋白A2(HSPA2)、胰岛素样生长因子结合蛋白(IGFBP-2)在胰腺癌患者中的表达水平及其与临床病理特征的相关性。方法选取本院2015年4月至2019年4月行手术切除的胰腺癌组织标本96例、癌旁正常胰腺组96例、胰腺良性肿瘤组织标本88例作为研究对象,检测3组HSPA2、IGFBP-2蛋白表达水平,分析HSPA2、IGFBP-2蛋白对胰腺癌诊断价值。并对比不同临床病理特征患者胰腺癌组织标本中HSPA2、IGFBP-2蛋白表达水平及与临床病理特征相关性。结果腺癌组织标本HSPA2、IGFBP-2蛋白阳性表达率高于胰腺良性肿瘤组织标本、癌旁正常胰腺组织标本,差异具有统计学意义(P<0.05);HSPA2、IGFBP-2蛋白表达与胰腺癌患者TNM分期、淋巴结转移呈正相关,与分化程度呈负相关(P<0.05);经ROC曲线分析,两者联合诊断AUC为0.800,大于HSPA2、IGFBP-2单一诊断。结论胰腺癌组织HSPA2、IGFBP-2蛋白异常高表达,并与TNM分期、淋巴结转移呈正相关,与分化程度呈负相关,监测其蛋白表达水平,可为临床判断病情发展提供诊断依据。Objective To investigate the expression levels of heat shock protein A2(HSPA2) and insulin-like growth factor binding protein(IGFBP-2)in patients with pancreatic cancer and their correlation with clinical pathological characteristics. Methods A total of 96 patients with pancreatic cancer tissues who underwent surgical resection from April 2015 to April 2019 in our hospital,96 patients with normal pancreas adjacent to the cancer group,and 88 patients with benign pancreatic tumor tissue samples were selected as the research objects. The expression levels of HSPA2 and IGFBP-2 proteins in three groups were detected,and the diagnostic value of HSPA2 and IGFBP-2 proteins in pancreatic cancer was analyzed. The expression levels of HSPA2 and IGFBP-2 in pancreatic cancer tissue samples from patients with different clinical pathological characteristics were compared and their correlation with clinical pathological characteristics. Results The positive expression rate of HSPA2 and IGFBP-2 protein in pancreatic cancer tissue specimens was higher than that in benign pancreatic tumor tissue specimens and normal pancreatic tissue specimens adjacent to the cancer,the difference was statistically significant(P<0.05). The expression of HSPA2 and IGFBP-2 protein was positively correlated with TNM stage and lymph node metastasis in patients with pancreatic cancer,and negatively correlated with the degree of differentiation(P<0.05). The combined diagnosis of AUC was 0.800,which was greater than the single diagnosis AUC of HSPA2 or IGFBP-2. Conclusions The abnormally high expression of HSPA2 and IGFBP-2 proteins in pancreatic cancer tissues is positively correlated with TNM stage and lymph node metastasis,and negatively correlated with the degree of differentiation. Monitoring the protein expression levels can provide a diagnostic basis for clinical judgment of disease development.

关 键 词：热休克蛋白A2 胰岛素样生长因子结合蛋白 胰腺癌 TNM分期 分化程度 肿瘤 

分 类 号：R735.9[医药卫生—肿瘤]