IL-17在乳牙根尖周病损组织中的表达  

Expression of IL-17 in Chronic Periapical Periodontitis of Deciduous Teeth

作　　者：周焱[1] 王颖慧 赵焕英[3] 尚佳健[1] ZHOU Yan;WANG Yinghui;ZHAO Huanying;SHANG Jiajian(Department of Pediatric Dentistry, Capital Medical University School of Stomatology, Beijing 100050, China;Department of Medical Imaging, Peking University Hospital of Stomatology, Beijing 100081, China;Medical Experiment and Test Center, Capital Medical University, Beijing 100069, China)

机构地区：[1]首都医科大学附属北京口腔医院儿童口腔科,北京100050 [2]北京大学口腔医学院口腔医院医学影像科,北京100081 [3]首都医科大学医学实验与检测中心,北京100069

出　　处：《口腔医学研究》2020年第4期365-368,共4页Journal of Oral Science Research

基　　金：首都医科大学附属北京口腔医院学科建设基金(16-09-15)。

摘　　要：目的:检测IL-17在乳牙根尖周病损组织中的表达和分布,分析其在不同病理类型及炎症程度之间的关系,探讨其在乳牙慢性根尖周炎发病机制中的可能作用。方法:收集120例乳牙慢性根尖周病损组织行常规组织病理学检查,确定病理类型并按炎症细胞浸润程度分级;免疫组织化学法检测组织中IL-17的分布特点;ELISA法检测IL-17的蛋白表达量。结果:120例乳牙慢性根尖周病损组织中根尖周肉芽肿占65.8%,根尖周囊肿占18.4%,根尖周脓肿占15.8%。IL-17在3种病理类型中均有表达,主要表达于淋巴细胞、浆细胞。ELISA结果显示IL-17在不同病理类型组中的表达均低于正常对照组,在根尖肉芽肿组中的表达与炎症程度呈负相关。结论:IL-17在乳牙根尖周病损组织内广泛存在,随炎症程度加重表达逐渐降低,推测IL-17在乳牙慢性根尖周炎的病程进展中可能发挥一定的抑制作用。Objective:To detect the expression and distribution of IL-17 in periapical lesions of deciduous teeth and investigate the possible role of IL-17 in the pathogenesis of chronic periapical periodontitis in deciduous teeth.Methods:Tissue samples were collected from 120 deciduous teeth with chronic periapical periodontitis.Histopathological examination was performed to determine the pathological type and the degree of inflammatory cell infiltration.The distribution characteristics and expressions of IL-17 in tissues were detected by immunohistochemistry and ELISA.Results:Periapical granulomas(65.8%)were the most common pathological type in 120 deciduous teeth,followed by periapical cyst(18.4%)and periapical abscess(15.8%).IL-17 was expressed in three pathological types,mainly in lymphocytes and plasma cells.ELISA results showed that the expressions of IL-17 in three pathological types were lower than that in control group and the degree of inflammation was negatively correlated with IL-17 in periapical granulomas.Conclusion:IL-17 was detected widely in periapical lesions of deciduous teeth.Expression of IL-17 gradually decreased with the inflammation progression.IL-17 may contribute to the inhibition of the progression in chronic periapical periodontitis.

关 键 词：白细胞介素-17 乳牙 慢性根尖周炎 根尖肉芽肿 

分 类 号：R78[医药卫生—口腔医学]