白细胞介素-32与广西肝癌高发地区肝癌家族聚集性的相关性  

Correlation between interleukin 32 and familial clustering of hepatocellular carcinoma in an HCC-epidemic region of Guangxi,China

作　　者：彭金林 吴继周[1] 李国坚[1] 吴健林[1] 席玉梅 李晓晴 王磊 PENG Jinlin;WU Jizhou;LI Guojian;WU Jianlin;XI Yumei;LI Xiaoqing;WANG Lei(Department of Infectious Diseases, the First Affiliated Hospital,Guangxi Medical University, Nanning 530021, China;College of Health and Rehabilitation, Chengdu University of Chinese Medicine, Chengdu 610075, China)

机构地区：[1]广西医科大学第一附属医院感染性疾病科,广西南宁530021 [2]成都中医药大学养生康复学院,四川成都610075

出　　处：《暨南大学学报（自然科学与医学版）》2020年第3期246-252,共7页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(30960170);广西教育厅重点课题基金项目(桂教201202ZD021)。

摘　　要：目的:探讨广西肝癌高发区白细胞介素-32(IL-32)和肝癌家族聚集性的关系.方法:在广西肝癌高发地区选取115例肝癌高发家族成员为肝癌高发家族组,115例无癌家族成员为无癌家族组,采用酶联免疫吸附试验(ELISA)测定研究对象血清IL-32水平.从两组研究对象的血清IL-32总体水平及不同人口学特征(民族、HBsAg携带情况、性别、年龄等)层面比较IL-32的表达差异,分析IL-32表达与广西肝癌高发地区肝癌家族聚集性的关系.结果:肝癌高发家族组血清总体IL-32表达水平高于无癌家族组(Z=-2.702,P=0.007),肝癌高发家族组男性、HbsAg阴性、年龄≤25岁、瑶族成员IL-32表达水平均高于无癌家族组(均P<0.05),肝癌高发家族组中,不同等级亲缘关系的成员IL-32表达无统计学差异(P=0.429),具有不同肝癌先证者例数的肝癌高发家族成员IL-32表达差别不明显(P=0.298).男性成员的肝癌罹患率远远大于女性成员.结论:肝癌高发家族成员IL-32表达水平升高与肝癌家族聚集性存在一定的相关性,其过度表达增加了罹患肝癌的易感性.Objective:To investigate the association between the human serum level of interleukin-32(IL-32)and the familial aggregation of hepatocellular carcinoma(HCC)in a HCC-epidemic region of Guangxi,China.Methods:A total of 230 individuals without liver cancer were enrolled,including 115 from families with high HCC risk(HCC family group)and 115 from cancer-free families(non-cancer family group).Enzyme-linked immunosorbent assay was used to determine serum IL-32 level in the peripheral blood of all subjects.IL-32 expression difference was compared between two groups from general serum IL-32 level and aspects of different demographic characteristics,namely,ethnicity,HBsAg status,gender and age.Thereafter,the relationship between IL-32 expression and familial aggregation of HCC in Guangxi was analyzed.Results:The overall IL-32 expression in the HCC family group was higher than that in the non-cancer family group(Z=-2.702,P=0.007).The serum IL-32 expression in males,HBsAg-negative carriers,family members below 25 years old,and the Yao population in the HCC family group was significantly higher than that in the non-cancer family group(P<0.05).In the HCC family group,no statistical differences were observed in IL-32 level among members with different blood kinship to the probands(P=0.429)and among members of families with different numbers of probands(P=0.298).The prevalence rate of HCC in males significantly increased compared with that in females.Conclusion:The increased IL-32 expression might be correlated with the familial aggregation of HCC in Guangxi,and its overexpression might be a susceptibility factor that contributes to the familial clustering of HCC in Guangxi,China.

关 键 词：细胞因子 白细胞介素-32(IL-32) 肝细胞癌 家族聚集性 

分 类 号：R735.7[医药卫生—肿瘤]