Periostin对接触性超敏反应中的中性粒细胞调控作用的影响  

Effect of Periostin on Neutrophil Regulation in Contact Hypersensitivity

作　　者：张钟元 姜美花[1] ZHANG Zhong-yuan;JIANG Mei-hua(Center for Stem Cell Biology and Tissue Engineering,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]中山大学中山医学院干细胞与组织工程研究中心,广东广州510275

出　　处：《中山大学学报（医学科学版）》2020年第3期363-369,共7页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(81770290)。

摘　　要：【目的】探讨在小鼠耳肿模型炎症反应发生早期Periostin对中性粒细胞的调控作用。【方法】每组取30只,肿耳模型采用DNFB(1-氟-2,4-二硝基苯)致敏剂分别涂抹野生型小鼠(WT)和Periostin-/-小鼠(POSTN-/-)。在造模后24 h,使用螺旋测微器分别进行耳廓厚度测量;免疫组化染色、流式细胞分析的方法检测耳廓组织炎症细胞以及中性粒细胞浸润情况;实时荧光定量PCR(Q-PCR)分析中性粒细胞相关细胞因子、趋化因子的表达水平。【结果】致敏剂致耳肿模型前2组小鼠耳朵厚度经测量无统计学差异(P>0.05),致敏后24 h经测量POSTN-/-组耳廓肿胀程度大于WT组(P<0.05);HE染色显示POSTN-/-组炎症细胞浸润数目多于WT组(均P<0.05);免疫组化染色和流式细胞分析显示耳肿组织中中性粒细胞浸润比率POSTN-/-组高于WT组(P<0.05);qRT-PCR检测耳肿组织中的中性粒细胞相关细胞因子和趋化因子表达水平POSTN-/-组高于WT组(均P<0.05)。【结论】Periostin在小鼠耳肿炎症反应早期减少了中性粒细胞的浸润从而一定程度参与抑制炎症反应发生发展。【Objective】To investigate the role of Periostin in regulating neutrophils at the early stage of swelling ear model.【Methods】Thirty animals were selected in each group and ear sensitization induced by DNFB(1-fluoro-2,4-dinitrobenzene)sensitizer in wild type mice(WT)and Periostin-/-mice(POSTN-/-),respectively.After 24 h modeling,the auricle thickness was measured using a spiral micrometer.Immunohistochemical staining and flow cytometry were used to detect inflammatory cells and neutrophil infiltration in the auricle tissue.Real-time fluorescence quantitative PCR(qPCR)analysis was used to measure the expression of the neutrophil related cytokines and chemokines.【Results】Before the sensitizer-induced ear swelling model,the ear thickness of the two groups of mice was not statistically different(P>0.05);and 24 hours after sensitization,the degree of ear swelling was greater in the POSTN-/-group than in the WT group(P<0.05);HE staining showed that the number of inflammatory cells infiltrated in the POSTN-/-group was greater than that in the WT group(both P<0.05);immunohistochemical staining and flow cytometry analysis showed that the neutro?phil infiltration ratio in ear tissue was higher in POSTN-/-group than in WT group(all P<0.05);both qPCR detected neutrophil-associated cytokine and chemokine expression levels in ear tissues were higher in POSTN-/-group than in WT group(all P<0.05).【Conclusion】Periostin reduced the infiltration of neutrophils in the early stage of inflammatory reac?tion of mouse ear swelling,which may play a role in inhibiting the development of inflammatory response.

关 键 词：PERIOSTIN 炎症反应 中性粒细胞 细胞浸润 

分 类 号：R36[医药卫生—病理学]