化学修饰mRNA在细胞重编程、组织工程和肿瘤基因治疗中的研究进展  

Research Progress of Chemically Modified mRNA in Cell Reprogramming,Tissue Engineering and Tumor Gene Therapy

作　　者：谢路远 盛小伍 周晓 姚超灵 许安吉 XIE Luyuan;SHENG Xiaowu;ZHOU Xiao;YAO Chaoling;XU Anji(University of South China,Hengyang,Hunan,421001,China;Central Laboratory,Hunan Cancer Hospital/the Affiliated Cancer Hospital of Xiangya School of Medicine,Central South University,Changsha,Hunan,410013,China)

机构地区：[1]南华大学,湖南衡阳421001 [2]湖南省肿瘤医院/中南大学湘雅医学院附属肿瘤医院中心实验室,湖南长沙410013

出　　处：《肿瘤药学》2020年第2期132-138,共7页Anti-Tumor Pharmacy

基　　金：国家自然科学青年基金(81903262);湖南省卫生计生委基金项目(B20180357);长沙市科技局(kq1801112)。

摘　　要：通过大量DNA载体(如质粒、腺病毒、腺相关病毒、反义寡核苷酸等)递送外源基因,从而替代缺陷基因,恢复体内正常功能的过程,被称为体内基因调控。病毒介导的基因传递是目前最成功和最有效的基因治疗方法之一,但由于存在严重的安全问题,寻找新的基因递送方法十分必要。近年来,化学修饰的mRNA(cmRNA)作为备选载体,越来越多被应用于体内的基因递送。本文阐述了采用cmRNA在细胞重编程、组织工程和肿瘤基因治疗中的研究进展。The delivery of foreign genes through a large number of DNA vectors(such as plasmids,adenoviruses,adeno-associated viruses,antisense oligonucleotides,etc.)to replace defective genes and restore normal functions in the body is called in vivo gene regulation.Virus-mediated gene delivery is one of the most successful and effective methods of gene therapy.However,due to the serious safety problems,it is necessary to find new methods of gene delivery.Recently,chemically modified mRNAs(cmRNA)have been increasingly used as alternatives for gene delivery in vivo.This article mainly described the research advances in cmRNA for cell reprogramming,tissue engineering and tumor gene therapy.

关 键 词：化学修饰mRNA 诱导多能干细胞 细胞分化 肿瘤基因治疗 

分 类 号：R730.5[医药卫生—肿瘤]