p53/CXCL12在亲水性钛表面微纳米形貌促进人牙周膜成纤维细胞成骨分化中的作用  

Effect of hydrophilicity of titanium with micro/nanotopographical surface on osteogenic differentiation of human periodontal ligament cells via p53/CXCL12

作　　者：林玉娇[1] 庄秀妹[2] 谢奕文[1] LIN Yujiao;ZHUANG Xiumei;XIE Yiwen(Department of Stomatology,Shantou Central Hospital,Shantou 515031,Guangdong Province,China)

机构地区：[1]汕头市中心医院口腔科,广东汕头515031 [2]中山大学附属孙逸仙纪念医院口腔科,广东广州

出　　处：《中国口腔种植学杂志》2019年第4期154-157,190,共5页Chinese Journal of Oral Implantology

基　　金：国家自然科学基金青年基金项目(81600899)。

摘　　要：目的:探讨p53/CXCL12在亲水性钛表面微纳米形貌促进人牙周膜成纤维细胞(periodontal ligament cells,PDLCs)成骨分化中的作用。方法:前期已采用阳极氧化法和喷砂碱热法构建相似微纳米形貌但亲水性不同钛表面,两组钛表面接种PDLCs,第7天时Western免疫印迹检测p53、CXCL12表达水平;小干扰RNA (Si-p53、SiCXCL12)转染PDLCs,检测分别敲低其表达后p53、CXCL12表达水平变化,比较碱性磷酸酶(ALP)活性,成骨标志物ALP、I型胶原(COL1)、成骨特异性转录因子(RUNX2) mRNA水平变化。采用SPSS13.0软件包对数据进行统计学分析。结果:相比阳极氧化组,亲水性更优喷砂碱热组PDLCs中p53表达降低,CXCL12表达升高。Sip53转染PDLCs后,p53表达降低,CXCL12表达升高。Si-CXCL12转染PDLCs,CXCL12水平降低,p53水平无明显变化,但ALP、COL1与RUNX2的mRNA水平均显著升高。结论:亲水性钛表面微纳米形貌可下调p53,促进CXCL12表达升高,进而促进PDLCs成骨分化。Objective:To investigate the effect of p53/CXCL12 on the osteogenic differentiation of periodontal ligament cells(PDLCs) induced by hydrophilicity of titanium with micro/nanotopographical surface.Methods: Anodic oxidation and sandblast-alkali heat methods were used to establish scale structures,which were cultured with PDLCs. Expressions of p53 and CXCL12 were examined by western blot at 7 day. After transfection of p53 or CXCL12 with siRNA, p53 and CXCL12 level were furthered detected,changes of alkaline phosphatase(ALP) activity and m RNA expressions of such osteogenic markers as ALP, collagen-I(COL1) and runt related transcription factor 2(RUNX2) were examined to detect osteogenic differentiation of PDLCs. Date was statistically analyzed with SPSS13.0 software package. Results: Compared with anodic oxidation surfaces, p53 expression of PDLCs on sandblast-alkali heat surface discs with well hydrophilicity was significantly decreased, while CXCL12 level increased. Transfected with Si-p53, p53 level was decreased and CXCL12 level increased. After transfection of Si-CXCL12, CXCL12 level was decreased, but no significant change of p53 level. However, ALP activity and ALP, COL1 and RUNX2 mRNA levels were significantly decreased. Conclusion: Hydrophilicity of titanium with micro/nanotopographical surface promotes osteogenic differentiation of PDLCs via p53/CXCL12.

关 键 词：亲水性 人牙周膜成纤维细胞 P53 CXCL12 成骨分化 微纳米形貌 

分 类 号：R782[医药卫生—口腔医学]