新疆哈萨克族食管鳞癌与长链非编码RNA表达的关系  

Expression of long non-coding RNAs in esophageal squamous cell carcinoma of Kazak nationality in Xinjiang

作　　者：古丽扎热叶·艾库拉[1] 谭遥[2] 帕力达·阿皮孜阿吉[1] GULZARIA·Aikula;TAN Yao;PALIDA·Apiziaji(Breast Radiotherapy Department of Xinjiang Medical University Affiliated Cancer Hospital,Urumqi 830011;Thoracoabdominal Radiotherapy Department of Xinjiang Medical University Affiliated Cancer Hospital,Urumqi 830011,Xinjiang,China)

机构地区：[1]新疆医科大学附属肿瘤医院乳腺放疗科,新疆乌鲁木齐830011 [2]新疆医科大学附属肿瘤医院胸腹放疗科,新疆乌鲁木齐830011

出　　处：《癌变．畸变．突变》2020年第3期194-197,202,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：新疆维吾尔自治区自然科学基金(2016D01C354)。

摘　　要：目的:对新疆哈萨克族食管鳞癌组织及癌旁组织长链非编码RNA(lnc RNA)表达谱进行分析,并探讨其与食管癌发生和发展的关系。方法:采用基因芯片技术,对4组食管鳞癌及其癌旁组织中差异表达的lnc RNA进行筛选,通过lnc RNA-m RNA共表达网络对靶基因进行预测,利用GO和KEGG数据库进行富集分析和通路分析,明确lnc RNA的功能。结果:癌组织和癌旁组织之间差异表达的lnc RNA共318个,与癌旁组织比较,癌组织中193个lnc RNA表达上调,125个lnc RNA表达下调(P<0.01)。富集分析表明共有5个GO类别,其中细胞成分有3个,生物过程有1个,分子功能有1个,均通过反式调控方式调控靶基因。通路分析表明这些差异表达的基因可能参与轴突导向信号通路和ECM受体相互作用途径信号通路。结论:新疆哈萨克族食管鳞癌组织中lnc RNA的表达水平与癌旁组织有明显差异,差异表达的lnc RNA可能参与食管癌的复杂调控网络,并与肿瘤发生和发展有一定的关系。OBJECTIVE: To analyze the expression of long non-coding RNA(lncRNA) in esophagealsquamous cell carcinoma(ESCC) and adjacent tissues of Kazakh nationality in Xinjiang. METHODS:Differentially expressed lnc RNAs in four groups of cancer and a control group were screened by gene chiptechnology, and the target genes were predicted by lnc RNA-m RNA co-expression network. GO and KEGGdatabases were used for enrichment and Pathway analyses to clarify the function of lnc RNA. RESULTS: Atotal of 318 lnc RNAs were differentially expressed between the cancer and control groups. The expressionlevels of 193 lnc RNAs were higher for the cancer than the control groups, and 125 lnc RNAs werelower. Enrichment analyses show that there were 5 GO categories, including 3 cellular components, 1 biological process and 1 molecular function, all of which were target genes regulated by trans-regulation. Pathway analyses show that these differentially expressed genes might be involved in two signalpathways, such as axonal guidance signal pathway and ECM receptor interaction pathway. CONCLUSION:Expression levels of lnc RNAs in Kazakh esophageal squamous cell carcinoma were significantly different fromthat of the control group. The differentially expressed lnc RNAs may be involved in the complex regulatorynetwork of cancer and has a certain relationship with tumorigenesis and progression.

关 键 词：哈萨克族食管鳞癌 长链非编码RNA 靶基因预测 基因芯片 

分 类 号：R730.2[医药卫生—肿瘤]