枇杷叶中三萜酸类成分抗糖尿病及其并发症的体外活性研究  

Study on in vitro activities of triterpene acids from leaf of Eriobotrya japonica against diabetes and its complications

作　　者：陈剑[1] 吴月娴[1] 吕寒[1] 简暾昱[1] 丁晓琴 李佳伟 刘艳[1] 任冰如[1] CHEN Jian;WU Yuexian;LYU Han;JIAN Tunyu;DING Xiaoqin;LI Jiawei;LIU Yan;REN Bingru(Jiangsu Provincial Service Center for Anti-diabetic Drugs Screening, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园)江苏省抗糖尿病药物筛选技术服务中心,江苏南京210014

出　　处：《植物资源与环境学报》2020年第3期78-80,共3页Journal of Plant Resources and Environment

基　　金：国家自然科学基金资助项目(81773885,81703224)。

摘　　要：糖尿病属于多病因的代谢性疾病,显著临床特征为慢性高血糖,并常伴有糖、脂肪和蛋白质代谢紊乱。随着患病时间延长,若病人体内代谢紊乱得不到很好地控制,容易产生心脑血管疾病、肾功能衰竭、肢体坏疽和昏迷等并发症[1]。中草药在治疗糖尿病上具有独特疗效[2],从中草药资源中寻找治疗糖尿病及其并发症的天然产物已成为研究热点[2]。In vitro inhibitory effects of six triterpene acids(including euscaphic acid,tormentic acid,corosolic acid,maslinic acid,oleanolic acid,and ursolic acid)from leaf of Eriobotrya japonica(Thunb.)Lindl.onα-glucosidase activity and production of nonenzymatic glycosylation products Amadori and AGEs were studied.The results show that the six triterpene acids have strong inhibitory effects onα-glucosidase activity,with semi-inhibitory concentration(IC50)of 0.76-35.45μg·mL-1,in which,ursolic acid has the strongest inhibitory effect.The six triterpene acids show inhibitory effects on Amadori formation,and the inhibitory effects decrease significantly with the decrease of mass concentration,in which,50μg·mL-1 euscaphic acid has the strongest inhibitory effect,with inhibition rate of 83.63%;however,the six triterpene acids have no evident inhibitory effect on AGEs formation,and the inhibition rate of 50μg·mL-1 euscaphic acid has the strongest inhibitory effect,but the inhibition rate is only 18.71%.In conclusion,triterpene acids from leaf of E.japonica have potential therapeutic effects on diabetes and its complications.

关 键 词：枇杷叶 三萜酸类成分 Α-葡萄糖苷酶 非酶糖化反应产物 

分 类 号：Q946.8[生物学—植物学] R284[医药卫生—中药学]