Isolation of DNA from Saliva and Cheek Cells Using Household Chemicals  

作　　者：Sanober Kafeel 

机构地区：[1]Dr.A.Q.Khan Institute of Biotechnology and Genetic Engineering(KIBGE),University of Karachi,Karachi 75270,Pakistan

出　　处：《Journal of Life Sciences》2020年第1期22-24,共3页生命科学（英文版）

摘　　要：Saliva is a feasible specimen used in sample collection for research purposes.It is reported that saliva typically contains^430,000 epithelial cells per mL.There are various cost-effective methods and commercial kits available which are used to extract DNA from saliva.However,this study aims to explore a simple,time-saving and cheap method of DNA extraction using daily life household chemicals without the help of any electronic equipment.This protocol utilizes pineapple juice which contains bromelain enzyme as a proteolytic agent and dishwashing liquid as a detergent to rupture the cell membrane and remove the contamination.It takes approximately 10-15 min for the completion of extraction procedure.Results have indicated the good quality of DNA by gel electrophoresis and good yield with purity of 1.7-1.8 optical density.Thus,this method serves as a simple,convenient,easy-to-handle,time-saving,cheap and less laborious way for the extraction of DNA in daily routine practices with a good precision of quality and quantity that could be used for diagnostic purposes.

关 键 词：SALIVA DNA extraction dishwashing liquid bromelain enzyme 

分 类 号：R73[医药卫生—肿瘤]