细胞膜片技术及其在牙周组织再生中的研究进展  

Research progress on cell sheet technology and its application in periodontal tissue regeneration

作　　者：何梦娇[1] 李丽生 陈玉玲[1] 骆凯[1] HE Mengji-ao;LI Lisheng;CHEN Yuling;LUO Kai(School and Hospital of Stomatology,Fujian Medical University&Key Laboratory of Stomatology,Fujian Province University,Fuzhou 350002,China;Department of Emergency Surgery,Fujian Provincial Hospital,Fuzhou 350001,China)

机构地区：[1]福建医科大学附属口腔医院福建省高校口腔医学重点实验室,福建福州350002 [2]福建省立医院急诊外科,福建福州350001

出　　处：《口腔疾病防治》2020年第7期458-462,共5页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金项目(81870766,81100761);福建省科技创新联合资金项目(2016Y9023);福建省卫生系统中青年骨干人才培养项目(2015-ZQN-ZD-28)。

摘　　要：目前临床上常规采用的牙周治疗方法尚不能完全实现完整的和有效的牙周组织再生。细胞膜片技术(cell sheet technology,CST)是一种无需支架材料的细胞移植方法,可保留完整的细胞外基质及细胞重要离子通道、生长因子受体等,保证了细胞间及细胞与胞外基质之间的相互作用。本文从细胞膜片的种类、构建方法,综述其在牙周组织再生领域的应用及研究进展。不同类型的种子细胞均可制备成单层细胞膜片、多层细胞膜片、细胞膜片片段及细胞膜片聚合体。其中,单层细胞膜片较容易破损,对操作者要求较高。多层细胞膜片可提高机械性,但其厚度需控制,以避免出现细胞坏死。细胞膜片片段可应用于牙槽骨和牙根牙骨质之间的狭窄空间,降低操作难度,也提高膜片机械性。细胞膜片聚合体是三维结构,可提供强有力的机械支持,提高细胞膜片的稳定性,但其生物活性的稳定性尚需进一步提高。在构建细胞膜片的方法中,羊膜基底膜的抗纤维化抗血管生成的生物属性决定了其很适合充当细胞培养的基质;温度敏感性培养皿法简单易行;维生素C连续诱导法可保留细胞表面的一些重要蛋白;磁力组织工程法可使细胞粘附力增加,更容易形成稳定的细胞膜片,以上方法各有特点。在临床应用中,单层细胞膜片多应用于直接移植到受区位点,构建牙周组织;同种或异种多层细胞膜片叠加后移植到受区位点;同种多层细胞膜片包裹支架材料后移植到受区位点,构建三维结构组织。总而言之,CST在牙周组织再生中表现出了巨大的潜力。At present,conventional periodontal treatment cannot achieve complete and effective periodontal tissue regeneration.Cell sheet technology(CST)is a kind of cell transplantation method without scaffold material that can maintain complete extracellular matrix,important ion channels of cells,growth factor receptors,etc.,and ensure the in-teraction between cells and the extracellular matrix.In this paper,the application and research progress of the cell sheet in the field of periodontal tissue regeneration are reviewed.Different types of seed cells can be prepared into monolayer cell sheet,multilayer cell sheet,cell sheet fragments and cell sheet polymers.Among them,the monalayer cell sheet is easily damaged and requires high deoperator;the multilayer cell sheet shows improved mechanical properties,but its thickness needs to be controlled to avoid cell necrosis.The cell sheet fragment can be used in the narrow space between the alveolar bone and root cementum to reduce the difficulty of operation and improve the mechanical properties of the cell sheet.Cell sheet polymers are three-dimensional structures that can provide strong mechanical support and improve the stability of the cell sheet,but the stability of their biological activity needs to be further improved.In methods for construction of the cell sheet,the antifibrosis and antiangiogenesis properties of the amniotic sheet have shown that this structure is suitable as the matrix of cell culture;the method of using a temperature-sensitive culture dish is simple and easy;continuous induction with vitamin C can retain some important proteins on the cell surface;and the magnetic tis-sue engineering method can increase cell adhesion and easily form a stable cell sheet.The above methods have their own characteristics.In clinical applications,monolayer cell sheet is mainly used for direct transplantation to the receiv-ing site to construct periodontal tissue;multilayer cell sheet of the same or different species overlap and are then trans-planted to the receiving site;a

关 键 词：细胞膜片技术 单层细胞膜片 多层细胞膜片 细胞膜片聚合体 牙周组织再生 牙槽骨再生 牙骨质再生 组织工程 牙周炎 细胞移植 

分 类 号：R78[医药卫生—口腔医学]