P16和P15在恶性黑色素瘤中的表达及其临床意义  

Expression and Clinical Significance of P16 and P15 in Malignant Melanoma

作　　者：陈铨栩 陈湾湾[1] 张普[1] 罗晓智 赵志光[1] Chen Quanxu;Chen Wanwan;Zhang Pu(The Second Affiliated Hospital of Wenzhou Medical University,Zhejiang 325027,China)

机构地区：[1]温州医科大学附属第二医院,325027

出　　处：《医学研究杂志》2020年第5期145-149,共5页Journal of Medical Research

摘　　要：目的探讨P16和P15在恶性黑色素瘤中的表达及其临床意义。方法采用SP法免疫组化检测66例恶性黑色素瘤组织中P16、P15的表达情况,统计患者的临床资料分析其表达与临床特征的相关性,采用Kaplan-Meier曲线对随访数据进行预后分析。结果恶性黑色素瘤中P16(χ^2=33.470,P=0.000)、P15(χ^2=51.603,P=0.000)的表达率均显著低于皮内痣组织。恶性黑色素瘤中P16的阳性表达水平与肿瘤浸润深度(χ^2=17.888,P=0.000)、淋巴结转移(χ^2=5.561,P=0.018)和Ki-67增殖指数(χ^2=7.108,P=0.008)呈负相关;P15的阳性表达水平与肿瘤浸润深度(χ^2=9.156,P=0.000)、淋巴结转移(χ^2=8.817,P=0.003)、远处转移(χ^2=4.970,P=0.026)和Ki-67增殖指数(χ^2=15.422,P=0.000)呈负相关。P16、P15表达阴性的患者,中位总体生存期明显短于表达阳性的患者(14.0个月vs 28.6个月,P=0.025;14.0个月vs 33.4个月,P=0.003)。结论P16和P15可能在恶性黑色素瘤的发生和发展中起负性作用,可以作为鉴别恶性黑色素瘤和良性黑素细胞病变的指标,也可能对MM患者的预后有一定的指导意义。Objective To investigate the expression and significance of P16 and P15 in malignant melanoma.Methods SP immunohistochemistry was used to detect the expression of P16 and P15 in 66 malignant melanoma.The clinical data were collected to analyze the relationship between the expression and clinical characteristics.Kaplan-Meier curves were used to conduct survival analysis of follow-up data.Results The expression of P16(χ^2=33.470,P=0.000)and P15(χ^2=51.603,P=0.000)in malignant melanoma were both significantly lower than those of intradermal nevus.There was negative correlation between the expression of P16 and tumor infiltration depth(χ^2=17.888,P=0.000),lymph node metastasis(χ^2=5.561,P=0.018)and Ki-67(χ^2=7.108,P=0.008),and there was negative correlation between the expression of P15 and tumor infiltration depth(χ^2=9.156,P=0.000),lymph node metastasis(χ^2=8.817,P=0.003),distant metastasis(χ^2=4.970,P=0.026)and Ki-67(χ^2=15.422,P=0.000).The median survival time of patients with negative expression of P16 and P15 was significantly shorter than that of patients with positive expression in malignant melanoma(14.0 months vs 28.6 months,P=0.025;14.0 months vs 33.4 months,P=0.003).Conclusion P16 and P15 may play a negative role in the occurrence and development of malignant melanoma.They can be used as a molecular marker for differential diagnosis of malignant melanoma and benign melanocyte lesions,and may be helpful for the prognosis of patients with malignant melanoma.

关 键 词：恶性黑色素瘤 P16 P15 预后分析 

分 类 号：R739.5[医药卫生—肿瘤]