姜黄素在小鼠肺成纤维细胞中的抗纤维化作用  

Antifibrotic Effects of Curcumin in Mouse Lung Fibroblasts

作　　者：龚玲 刘代顺 朱红兰 GONG Ling;LIU Dai-shun;ZHU Hong-lan(Department of Respiratory Medicine,The Third Affiliated Hospital of Zunyi Medical University(The First People’s Hospital of Zunyi),Guizhou Zunyi 563002,China)

机构地区：[1]遵义医科大学第三附属医院(遵义市第一人民医院呼吸内科),贵州遵义563002

出　　处：《中国中医基础医学杂志》2020年第4期483-488,共6页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：中国医药教育协会孙思邈中医药研究专项课题(2616SKT-M015)-姜黄素与PRAR-γ/PDGF-β在肺纤维化中的实验研究;贵州省卫生健康委科学技术基金项目(gzwjk2019-1-088)-探讨IL-32调控HDAC/ER Stress/EMT在肺纤维化发病机制中的影响。

摘　　要：目的:探讨姜黄素在小鼠肺成纤维细胞中的抗纤维化作用。方法:体外培养C57BL/6小鼠肺成纤维细胞,以TGF-β210ng/ml刺激成纤维细胞分化,使用不同浓度姜黄素抑制成纤维细胞分化,MTT及镜下检测C57BL/6小鼠肺成纤维细胞活力、增殖,PCR检测PPAR-γmRNA、PDGFR-βmRNA转录水平,Western blotting检测PPAR-γ、PDGFR-β蛋白表达,ELISA检测胶原蛋白-1表达。结果:姜黄素抑制C57BL/6小鼠肺成纤维细胞活力、增殖,增加PPAR-γmRNA转录水平及抑制PDGFR-βmRNA转录水平,增加PPAR-γ蛋白及抑制PDGFR-β蛋白表达,抑制胶原蛋白-1表达。结论:姜黄素抑制小鼠肺成纤维细胞纤维化形成。Objective:To explore the effect of curcumin in mouse lung fibroblasts.Methods:Mouse lung fibroblasts C57BL/6 was cultured in vivo,stimulated with transforming growth factor-β210ng/ml,and inhibited the transition by different concentrations of curcumin(5μmol/L,25μmol/L,50μmol/L).3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)colorimetric assay and microscopic were utilized to observe the vitality and proliferation of mouse lung fibroblasts C57BL/6.PCR was used to detect the mRNA expression of PPAR-γand PDGFR-β.Western blotting was performed to observe the protein expression of PPAR-γand PDGFR-β.ELISA assay was employed to detect the expression of collagen-1.Results:Curcumin inhibited the vitality and proliferation of mouse lung fibroblasts C57BL/6.The increased expression of PPAR-γin mRNA level and the decreased expression of PDGFR-βin mRNA level were observed.Curcumin could promote the protein expression of PPAR-γand inhibit the protein expression of PDGFR-β.Additionally,curcumin could inhibit the expression of collagen-1.Conclusion:Curcumin inhibits fibrogenesis in mouse lung fibroblasts.

关 键 词：姜黄素 肺纤维化 转化生长因子Β2 PPAR-Γ PDGFR-Β 

分 类 号：R285.5[医药卫生—中药学]