淋巴瘤合并感染患者中TH1/TH2细胞因子和淋巴细胞亚群的变化  

Analysis of TH1/TH2 cytokines and lymphocyte subsets in patients with lymphoma co-infection

作　　者：温珍珍 李铭杰 刘亚楠 王宏 关则兵 潘学谊[1] WEN Zhenzhen;LI Mingjie;LIU Yanan;WANG Hong;GUAN Zebing;PAN Xueyi(Department of Hematology,The Frist Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou 510080,China)

机构地区：[1]广东药科大学附属第一医院血液科,广东广州510080

出　　处：《广东药科大学学报》2020年第3期426-430,共5页Journal of Guangdong Pharmaceutical University

基　　金：广东省自然科学基金项目(2017A030313664);广州市越秀区科技计划项目(2017-WS-008)。

摘　　要：目的探讨TH1/TH2细胞因子和淋巴细胞亚群在淋巴瘤合并感染患者中的变化情况。方法收集121例恶性淋巴瘤合并感染患者(感染组)和94例单纯淋巴瘤患者(对照组),检测两组患者的TH1/TH2细胞因子[白介素(IL)-2(IL-2)、IL-4、IL-6、IL-10、肿瘤坏死因子-α(TNF-α)和干扰素-γ(INF-γ)]的表达,以及淋巴细胞亚群(CD3+、CD4+、CD8+、CD19+、CD4/CD8、CD16/56+)分布情况,并进行比较分析。结果① 经H检验结果显示,抗感染治疗前,感染组TH1/TH2细胞因子表达水平高于对照组(P<0.05,P<0.01)。感染组两两比较,细菌感染者IL-6、IL-10、INF-γ、TNF-α表达水平高于病毒感染、真菌感染者(P<0.05,P<0.01),G+菌感染者的IL-6、IL-10表达水平高于G-菌感染者(P<0.05,P<0.01),其余指标比较差异无统计学意义(P>0.05)。② 抗感染治疗前,与对照组相比,感染组CD3+、CD4+、CD4/CD8、CD16/56+偏低,CD8+、CD19+偏高(P<0.05,P<0.01);感染组内两两比较,差异无统计学意义(P>0.05)。③ 感染组治疗前后,IL-2、TNF-α表达水平及CD3+、CD4+、CD19+、CD4/CD8、CD16/56+分布情况差异无统计学意义(P>0.05);抗感染治疗7 d后,IL-4、IL-6、IL-10、INF-γ表达水平及CD8+下降(P<0.05,P<0.01)。结论TH1/TH2细胞因子和淋巴细胞亚群在淋巴瘤、淋巴瘤合并感染患者中,具有一定的差异性,对淋巴瘤患者是否合并感染及感染的治疗效果具有一定的鉴别价值。Objective To investigate the changes of TH1/TH2 cytokines and lymphocyte subsets in patients with lymphoma coinfection.Methods 121 cases of malignant lymphoma with infection(infection group)and 94 cases of simple lymphoma(control group)were collected to study,and the expression of TH1/TH2 cytokines IL-2,IL-4,IL-6,IL-10,tumor necrosis factor-α(TNF-α)and interferon-γ(INF-γ)and lymphocyte subsets(CD3+,CD4+,CD8+,CD19+,CD4/CD8,and CD16/56+)in two groups of patients were detected and analyzed.Results①The results of H test showed that the expression of TH1/TH2 cytokines in infection group was higher than that in control group before anti-infection treatment(P<0.05,P<0.01).The levels of IL-6,IL-10,INF-γand TNF-αin bacterial infection group were higher than that in viral and fungal infection group(P<0.05,P<0.01).The levels of IL-6 and IL-10 in patients with G+bacterial infection were higher than that in patients with G-bacterial infection(P<0.05,P<0.01).There was no significant difference in other indicators.②Compared with the control group,CD3+,CD4+,CD4/CD8 and CD16/56+lymphocytes in the infection group were lower,but CD8+and CD19+lymphocytes were higher before anti-infection treatment P<0.05,P<0.01).There was no significant difference between the infected groups(P>0.05).③There was no significant difference in the expression of IL-2 and TNF-αand the distribution of CD3+,CD4+,CD19+,CD4/CD8 and CD16/56+lymphocytes in the infection group before and after treatment(P>0.05).After anti-infection treatment for 7 days,the expression level of IL-4,IL-6,IL-10,INF-γand CD8+lymphocytes were decreased(P<0.05,P<0.01).Conclusion Th1/Th2 cytokines and lymphocyte subsets have certain differences in patients with lymphoma and lymphoma combined with infection,which has a certain differential value for whether lymphoma patients combined with infection and the treatment effect of infection.

关 键 词：恶性淋巴瘤 感染 TH1/TH2细胞因子 淋巴细胞亚群 

分 类 号：R733[医药卫生—肿瘤]