MMP2、TIMP2、Ki-67、P53在胶质瘤组织中的表达及意义  

Expressions of MMP2,TIMP2,Ki-67 and P53 in glioma tissues and their significance

作　　者：于学娟[1] 安宏伟 孙亚梅 姜政[4] 张学海[5] 杨文敬 张伟[6] Yu Xuejuan;An Hongwei;Sun Yamei;Jiang Zheng;Zhang Xuehai;Yang Wenjing;Zhang Wei(Department of Radiation Oncology,Qilu Hospital of Shandong University,Jinan 250012,China;Department of Neurosurgery,Lingcheng District Hospital of Traditonal Chinese Medicine of Dezhou,Shandong Province,Dezhou 253500,China;Department of Health Management,Zhucheng People′s Hospital,Shandong Province,Zhucheng 262200,China;Department of Neurosurgery,Qilu Hospital of Shandong University,Jinan 250012,China;Department of Intensive Care Unit,Qilu Hospital of Shandong University,Jinan 250012,China;Department of Neurosurgery,Yidu Central Hospital of Weifang,Shandong Province,Weifang 262500,China)

机构地区：[1]山东大学齐鲁医院放疗科,济南250012 [2]山东省德州市陵城区中医院神经外科,253500 [3]山东省诸城市人民医院健康管理科,262200 [4]山东大学齐鲁医院神经外科,济南250012 [5]山东大学齐鲁医院重症监护室,济南250012 [6]山东省潍坊市益都中心医院神经外科,262500

出　　处：《国际肿瘤学杂志》2019年第12期718-722,共5页Journal of International Oncology

基　　金：山东省自然科学基金(ZR2014HM074)。

摘　　要：目的 探讨基质金属蛋白酶2(MMP2)、组织金属蛋白酶抑制物2(TIMP2)、Ki-67、P53在脑胶质瘤组织中的表达及意义。方法 回顾性分析山东大学齐鲁医院1995年1月-2015年12月期间的50例胶质瘤石蜡标本中Ki-67、P53表达(免疫组织化学SP法)情况,用免疫组织化学法检测胶质瘤组织MMP2、TIMP2的表达,运用χ^2检验分析不同病理分级各指标之间的差异,MMP2、TIMP2与Ki-67、P53的相关性分析采用Spearman等级相关分析法。结果 在胶质瘤高级别组(Ⅲ~Ⅳ级,n=37)中MMP2的高表达率为81.08%(30/37),Ki-67、P53的阳性表达率分别为78.38%(29/37)、72.97%(27/37),低级别组(Ⅰ~Ⅱ级,n=13)中MMP2高表达患者2例,Ki-67、P53的阳性表达患者均为3例,差异均具有统计学意义(χ^2=15.282,P<0.001;χ^2=10.482,P=0.001;χ^2=9.979,P=0.002),且均与病理分级呈正相关(r=0.600,P<0.001;r=0.505,P<0.001;r=0.447,P=0.001);TIMP2高表达在低级别组为8例,高级别组为19例(51.35%),差异无统计学意义(χ^2=0.402,P=0.526)。MMP2与Ki-67、P53均呈正相关(r=0.392,P=0.005;r=0.323,P=0.022);TIMP2与Ki-67呈负相关(r=-0.441,P=0.001);P53与Ki-67呈正相关(r=0.748,P<0.001)。结论 MMP2、Ki-67及P53可能在胶质瘤的增殖、侵袭过程中有重要作用;而TIMP2机制复杂,有待进一步研究。Objective To investigate the expressions and significance of matrix metalloproteinase 2(MMP2),tissue inhibitor of metalloproteinase 2(TIMP2),Ki-67 and P53 in human glioma tissues.Methods The expressions of Ki-67 and P53 in paraffin samples of 50 gliomas(immunohistochemistry SP method)from January 1995 to December 2015 in Qilu Hospital of Shandong University were analyzed retrospectively,and the expressions of MMP2 and TIMP2 were detected by immunohistochemistry.The differences of parameters between high-and low-grade gliomas were compared byχ^2 test and their correlations were assessed by Spearman correlation analysis.Results In the high-grade group(gradeⅢ-Ⅳ,n=37),the high expression rate of MMP2 was 81.08%(30/37),the positive expression rates of Ki-67 and P53 were 78.38%(29/37)and 72.97%(27/37).In the low-grade group(gradeⅠ-Ⅱ,n=13),there were 2 patients with high expression of MMP2,3 patients with positive expression of Ki-67 and P53 respectively,and there were significant differences between the two groups(χ^2=15.282,P<0.001;χ^2=10.482,P=0.001;χ^2=9.979,P=0.002).A significant correlation was found between them and pathological grade(r=0.600,P<0.001;r=0.505,P<0.001;r=0.447,P=0.001).The high expression of TIMP2 was found in 8 cases of low-grade group and 19 cases(51.35%)of high-grade group,with no significant difference(χ^2=0.402,P=0.526).The expression of MMP2 was positively correlated with Ki-67 and P53(r=0.392,P=0.005;r=0.323,P=0.022),while TIMP2 was negatively correlated with Ki-67(r=-0.441,P=0.001).The expression of P53 was positively correlated with Ki-67(r=0.748,P<0.001).Conclusion MMP2,Ki-67 and P53 may play important role in the proliferation and invasiveness of glioma.The mechanism of TIMP2 is complicated and needs further study.

关 键 词：神经胶质瘤 基质金属蛋白酶2 基质金属蛋白酶抑制剂2 KI-67抗原 肿瘤抑制蛋白质P53 

分 类 号：R73[医药卫生—肿瘤]