Transcytosis of Junonia coenia densovirus VP4 across the gut epithelium of Spodoptera frugiperda(Lepidoptera:Noctuidae)  

作　　者：Mariah Kemmerer Bryony C.Bonning 

机构地区：[1]Department of Entomology,Iowa State University,Ames,Iowa,USA [2]Department of Entomology and Nematology,University of Florida,Gainesville,Florida,USA

出　　处：《Insect Science》2020年第1期22-32,共11页昆虫科学（英文版）

基　　金：supported by the National Science Foundation Industry/University Cooperative Research Center,the Center for Arthropod Management Technologies under Grant No.IIP-1338775,and by industry partners.

摘　　要：The Junonia coenia densovirus rapidly traverses the gut epithelium of the host lepidopteran without replicating in the gut cells.The ability of this virus to transcytose across the gut epithelium is of interest for the potential use of virus structural proteins as delivery vehicles for insecticidal peptides that act within the insect hemocoel,rather than in the gut.In this study,we used fall armyworm,Spodoptera frugiperda to examine the binding of the virus to brush border membrane vesicle proteins by two-dimensional ligand blot analysis.We also assessed the rate of flux of the primary viral structural protein,VP4 fused to eGFP with a proline-rich linker(VP4-P-eGFP)through the gut epithelium ex vivo in an Ussing chamber.The mechanisms involved with transcytosis of VP4-P-eGFP were assessed by use of inhibitors.Bovine serum albumin(BSA)and eGFP were used as positive and negative control proteins,respectively.In contrast to BSA,which binds to multiple proteins on the brush border membrane,VP4-P-eGFP binding was specific to a protein of high molecular mass.Protein flux was significantly higher for VP4-P-eGFP after 2 h than for albumin or eGFP,with rapid transcytosis of VP4-P-eGFP within the first 30 min.In contrast to BSA which transcytosed following clathrin-mediated endocytosis,the movement of VP4-P-eGFP was vesicle-mediated but clathrin-independent.The specificity of binding combined with the efficiency of transport across the gut epithelium suggest that VP4 will provide a useful carrier for insecticidal peptides active within the hemocoel of key lepidopteran pests including S.frugiperda.

关 键 词：BSA gut epithelium JcDNV LEPIDOPTERA TRANSCYTOSIS virus 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]