日本沼虾养殖过程中氨基脲存在特征研究  

Study on the characteristics of semicarbazide(SEM)in Macrobrachium nipponense during the culture period

作　　者：舒秀君 程波[2] 徐娟娟 熊贻伟[3] 宋蓓[2] 宋怿[2] 韩刚[2] 蒋速飞[3] SHU Xiu-jun;CHENG Bo;XU Juan-juan;XIONG Yi-wei;SONG Bei;SONG Yi;HAN Gang;JIANG Su-fei(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Quality and Standard Research Centre,Chinese Academy of Fishery Sciences,Key Laboratory of Control of Quality and Safety for Aquatic Products,Ministry of Agriculture,Beijing 100141,China;Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院质量与标准研究中心,农业农村部水产品质量安全控制重点实验室,北京100141 [3]中国水产科学研究院淡水渔业研究中心,江苏无锡214081

出　　处：《淡水渔业》2020年第3期11-16,共6页Freshwater Fisheries

基　　金：国家自然科学青年基金项目(31502191);现代农业产业技术体系专项资金(CARS-48);中央级公益性科研院所基本科研业务费(2016C009)。

摘　　要：为探究不同生长时期养殖日本沼虾(Macrobrachium nipponense)体内氨基脲(Semicarbazide,SEM)的存在特征与变化规律,从养殖池塘采集不同生长时期日本沼虾样品,对各组织中SEM含量进行测定。结果表明:不同生长阶段日本沼虾样品中均有SEM检出,肌肉、甲壳、附肢、眼柄、鳃、肝胰腺中总SEM含量分别为(6.88±0.15)^(17.11±0.62)、(107.30±1.10)^(306.09±8.69)、(243.90±8.55)^(532.31±26.27)、(85.27±3.19)^(122.82±2.60)、(48.43±2.13)^(85.60±1.33)、(13.51±1.15)^(25.94±1.78)μg/kg,呈现:附肢>甲壳>眼柄>鳃>肝胰腺>肌肉的含量分布特征。SEM在肌肉和肝胰腺中主要以游离态形式存在,游离态SEM所占平均比例分别为67.35%和72.4%,在甲壳、眼柄、附肢、头胸部和鳃中主要以结合态形式存在,结合态SEM所占平均比例分别为89.50%、87.16%、85.68%、80.48%和73.30%。随着养殖过程进行,肌肉中总SEM含量总体逐渐降低,甲壳和附肢中总SEM含量呈现总体上升的变化趋势。研究结果可为甲壳类水产品中SEM残留限量标准的修订和采样技术规范的制订提供基础数据。Samples of Macrobrachium nipponense at different growth stages were collected from an aquaculture pond and determined the concentrations of SEM to explore the characteristics and variation rules of semicarbazide(SEM)in cultured M.nipponense of different growth stages.Results indicated that SEM were detected in all the samples.The concentrations of total SEM in muscle,shell,pereopod,eyestalks,gill and hepatopancreas were(6.88±0.15)-(17.11±0.62),(107.30±1.10)-(306.09±8.69),(243.90±8.55)-(532.31±26.27),(85.27±3.19)-(122.82±2.60),(48.43±2.13)-(85.60±1.33)and(13.51±1.15)-(25.94±1.78)μg/kg,respectively.The distribution characteristics of total SEM were showed as pereopod>shell>eyestalks>gill>hepatopancreas>muscle.SEM mainly existed in the free form in muscle and hepatopancreas of M.nipponense,and the average proportion of free SEM was 67.35%and 72.4%.In the shell,eyestalks,pereopod,cephalothorax and gill,SEM mainly existed in the tissue-bound form,the average proportion of tissue-bound SEM was 89.50%,87.16%,85.68%,80.48%and 73.30%,respectively.With the culture processed,the concentration of total SEM in muscle of M.nipponense overall decreased gradually,and it showed an overall upward trend in the shell and pereopod.The research results provided basic data for the revision of the SEM residue limit standards in crustacean aquatic products and the establishment of sampling technical specifications.

关 键 词：日本沼虾(Macrobrachium nipponense) 氨基脲 存在形态 分布特征 变化规律 

分 类 号：TS207.3[轻工技术与工程—食品科学]