组蛋白甲基化酶EZH2及VEGF165在烟雾病中的作用研究  

Role of histone methylation enzyme EZH2 and VEGF165 in momymoya disease

作　　者：郭再玉[1] 张合亮[1] 陈谦[1] 侯延伟 水涛[1] Guo Zaiyu;Zhang Heliang;Chen Qian;Hou Yanwei;Shui Tao(Department of Neurosurgery,Tianjin TEDA Hospital,Tianjin 300457,China)

机构地区：[1]天津市泰达医院神经外科,300457

出　　处：《国际生物医学工程杂志》2019年第6期479-483,共5页International Journal of Biomedical Engineering

基　　金：滨海新区卫生局医药卫生科技项目(2011BHKZ007)。

摘　　要：目的探讨组蛋白甲基化酶zeste基因增强子同源物2(EZH2)及血管内皮生长因子165(VEGF 165)在烟雾病中的作用。方法选取新西兰大耳白兔,采用耳静脉注射马血清的方法建立烟雾病动物模型,并通过包装慢病毒的方法原位过表达VEGF165,采用实时荧光定量PCR和蛋白质印迹法(Western Blot)检测大耳白兔脑组织中VEGF165?EZH2和H3K27me3的表达变化。结果与正常对照组相比,烟雾病模型组EZH2的mRNA和蛋白表达水平升高(EZH2 mRNA:P<0.01),组蛋白H3K27me3水平升高;在烟雾病模型中过表达VEGF165后,EZH2的mRNA和蛋白表达水平进一步升高(EZH2 mRNA:P<0.01),组蛋白H3K27me3水平亦升高。结论EZH2在烟雾病的发病过程中起到了一定作用,且EZH2的表达受VEGF165的调控,这为烟雾病发病机理的研究提供了理论基础。Objective To study on the role of histone methylation enzyme enhancer of zeste homolog 2(EHZ2)and vascular endothelial growth factor 165(VEGF165)in momymoya disease.Methods The animal model of moyamoya disease was established by ear vein injection of horse serum in New Zealand rabbits.VEGF165 was over-expressed in situ by packaging lentivirus.Real-time quantitative PCR and Western Blot were used to detect the expression of VEGF165,EZH2 and H3K27me3 in the brain tissues of the animal models.Results Compared with the normal control group,the expression levels of mRNA and protein of EZH2 in the moyamoya disease model group were increased(EZH2 mRNA:P<0.01),and the level of histone H3K27me3 was increased.After overexpression of VEGF165 in the moyamoya disease model group,the expression levels of mRNA and protein of EZH2 was further increased(EZH2 mRNA:P<0.01),and the level of histone H3K27me3 was also increased.Conclusions EZH2 plays a certain role in the pathogenesis of moyamoya disease,and the expression of EZH2 is regulated by VEGF 165,which provides a theoretical basis for the study of the pathogenesis of moyamoya disease.

关 键 词：脑底异常血管网病 zeste基因增强子同源物2 血管内皮生长因子165 

分 类 号：R74[医药卫生—神经病学与精神病学]