肌动蛋白家族成员C1在肝细胞癌中的表达及其与预后和临床病理特征的相关性  

Expression of kinesin family member C1 in hepatocellular carcinoma and its correlation with prognosis and clinicopathological features

作　　者：刘义[1] 范华 王会会 张贝克[4] 翟景明[4] 范永刚[4] Liu Yi;Fan Hua;Wang Huihui;Zhang Beike;Zhai Jingming;Fan Yonggang(Department of Clinical Medicine,Henan University of Science and Technology,Luoyang 471003,China;Department of Graduate Students,the First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003,China;Department of General Medicine,the First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003,China;Department of Hepatobiliary and Pancreatic Surgery,the First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471003,China)

机构地区：[1]河南科技大学临床医学院,洛阳471003 [2]河南科技大学第一附属医院研究生科,洛阳471003 [3]河南科技大学第一附属医院全科医学科,洛阳471003 [4]河南科技大学第一附属医院肝胆胰外科,洛阳471003

出　　处：《国际生物医学工程杂志》2019年第6期484-489,共6页International Journal of Biomedical Engineering

摘　　要：目的研究肌动蛋白家族成员C1(KIFC1)在肝细胞癌(HCC)中的表达,分析其与患者临床病理特征及预后的相关性。方法通过免疫组化染色法测定KIFC1蛋白在82例HCC组织中的表达水平。分析KIFC1蛋白与临床病理特征(年龄、性别、肿瘤结节、肿瘤分级、肿瘤体积、淋巴结转移及甲胎蛋白表达)的相关性。通过Kaplan-Meier分析,分析HCC患者中KIFC1表达水平对总生存期和无进展生存期的影响。通过GPEIA数据库分析KIFC1的mRNA在肝癌组织中的表达水平。通过KM-plotter分析KIFC1表达与预后相关性。结果KIFC1蛋白在肝癌组织中显著高表达,其表达水平与肿瘤结节数(P=0.023)及肿瘤大小(P=0.011)有显著的相关性。KIFC1高表达的HCC患者的疾病总生存期和无病生存期均较短(均P<0.05)。KIFC1的mRNA在肝癌组织中显著高表达,并与患者无病生存率及总生存率相关。结论KIFC1蛋白在肝癌组织中异常高表达,其表达水平与肝癌的临床病理特征相关,生物信息学分析结果显示其与患者不良预后相关,提示KIFC1有望成为潜在的肝癌预后的预测因子和治疗靶点。Objective To study the expression of kinesin family member C1(KIFC1)in hepatocellular carcinoma(HCC)and analyze its correlation with the clinicopathological features and prognosis of patients.Methods The expression levels of KIFC1 protein in the HCC tissues from 82 patients were determined by immunohistochemical staining.The correlation between KIFC1 protein and clinicopathological characteristics(including age,gender,tumor nodules,tumor grade,tumor volume,lymph node metastasis,and alpha-fetoprotein expression)was analyzed.The Kaplan-Meier analysis was used to analyze the effect of KIFC1 expression level on overall survival and progression-free survival in patients with HCC.The expression level of KIFC1 mRNA in liver cancer tissue was analyzed by GPEIA database.The correlation between KIFC1 expression and prognosis was analyzed by KM-plotter.Results KIFC1 protein is significantly overexpressed in liver cancer tissues,and its expression level is significantly correlated with tumor nodule number(P=0.023)and tumor size(P=0.011).Patients with high expression of KIFC1 had poor overall disease and disease-free survival(all P<0.05).KIFC1 mRNA is significantly overexpressed in liver cancer tissues and correlated with disease-free survival and overall survival.Conclusions The expression of KIFC1 protein is highly expressed in liver cancer tissues,and its expression level is related to the clinicopathological characteristics of liver cancer.Bioinformatics analysis results show that KIFC1 is related to the poor prognosis of patients,suggesting that KIFC1 is expected to be a potential predictor and therapeutic target for liver cancer prognosis.

关 键 词：肝癌 肌动蛋白家族成员C1 免疫组化 临床病理特征 预后 

分 类 号：R735[医药卫生—肿瘤]