高效液相色谱法同时测定北败酱草中6个成分的含量  

Simultaneous determination of six components in Sonhi Arvensis Herba by HPLC

作　　者：刘志浩 宋平顺 杨平荣 张春江[3] LIU Zhi-hao;SONG Ping-shun;YANG Ping-rong;ZHANG Chun-jiang(School of Pharmacy,Lanzhou University,Lanzhou 730030,China;Gansu Institute of Drug Control,Gansu Province Chinese and Tibetan Medicine Inspection and Testing Technology Engineering Laboratory,Lanzhou 730070,China;College of Life Sciences,Lanzhou University,Lanzhou 730030,China)

机构地区：[1]兰州大学药学院,兰州730030 [2]甘肃省药品检验研究院,甘肃省中藏药检验检测技术工程实验室,兰州730070 [3]兰州大学生命科学院,兰州730030

出　　处：《药物分析杂志》2020年第4期607-612,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：甘肃省科技重大专项计划(17ZD2FA009)。

摘　　要：目的:建立同时测定北败酱草中绿原酸、秦皮乙素、咖啡酸、木犀草苷、木犀草素和芹菜素6个成分含量的方法,为制定质量标准提供依据。方法:采用高效液相色谱-二极管阵列检测法,使用Waters Symmetry C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.4%磷酸水溶液为流动相,进行梯度洗脱,流速1.0 mL·min^-1,柱温35℃,检测波长340 nm。结果:采用所建立的方法,6个成分分离度良好,平均加样回收率为96.8%~104.9%,RSD为0.6%~2.6%,符合定量分析要求。10批样品中绿原酸、秦皮乙素、咖啡酸、木犀草苷、木犀草素和芹菜素6个成分含量范围分别为148.0~3185.4、7.9~17.9、5.0~175.1、16.3~1411.5、24.3~2367.7、5.6~74.8μg·g^-1,含量差异较大,其中绿原酸、木犀草素和木犀草苷可作为北败酱草质量控制的指标性成分。结论:建立的分析方法适用于北败酱草的质量控制与评价。Objective:To establish a method for simultaneous determination of six components of chlorogenic acid,quercetin,caffeic acid,luteoloside,luteolin and apigenin in Sonhi Arvensis Herba,and to provide a basis for the establishment of quality standards.Methods:High performance liquid chromatography-photodiode array(HPLC-PDA)method was used.The separation was performed on Waters Symmetry C18 column(250 mm×4.6 mm,5μm).The mobile phase consisted of methanol-0.4% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL·min-1.The detection wavelength was 340 nm,and the column temperature was 35℃.Results:The six components were well separated,the average recovery rates were 96.8%-104.9% with RSDs of 0.6%-2.6%,which met the requirements of quantitative analysis.The content ranges of chlorogenic acid,quercetin,caffeic acid,luteoloside,luteolin and apigenin in 10 samples were 148.0-3185.4μg·g^-1,7.9-17.9μg·g^-1,5.0-175.1μg·g^-1,16.3-1411.5μg·g^-1,24.3-2367.7μg·g^-1 and 5.6-74.8μg·g^-1,respectively.The contents of the six components in 10 samples varied greatly,and chlorogenic acid,luteolin and luteoloside could be used as the index component to evaluate the quality of Sonhi Arvensis Herba.Conclusion:The established analytical method is suitable for the quality control and evaluation of Sonhi Arvensis Herba.

关 键 词：北败酱草 全叶苦苣菜 苦苣菜 药食两用植物 绿原酸 秦皮乙素 咖啡酸 木犀草苷 木犀草素 芹菜素 高效液相色谱法 含量测定 质量控制 

分 类 号：R917[医药卫生—药物分析学]