枸骨叶水提物对小鼠肥胖的预防作用及对脂肪分化的影响  

Preventive effect of Ilex cornuta aqueous extract on obese mice and its effects on adipose differentiation

作　　者：刘梅芳[1] 孙悦 李丽[1] LIU Mei-fang;SUN Yue;LI Li(College of Pharmacy,Jining Medical University,Rizhao 276826,China)

机构地区：[1]济宁医学院药学院,山东日照276826

出　　处：《中国病理生理杂志》2020年第5期899-905,共7页Chinese Journal of Pathophysiology

基　　金：国家级大学生创新训练计划项目(No.201610443038);济宁医学院大学生创新训练计划项目(No.cx2016038)。

摘　　要：目的:研究枸骨叶水提物(ICAE)对高脂饮食(HFD)小鼠肥胖的预防作用及对脂肪分化的影响.方法:本研究采用HFD诱导小鼠肥胖,同时灌胃给予ICAE,以奥利司他(orlistat)为阳性对照药。39只昆明雄性小鼠分成4组,包括对照组(n=10)、肥胖模型组(n=9)、orlistat治疗组(n=10)和ICAE治疗组(n=10)。以体重、腹内和皮下脂肪含量、肝重以及血清甘油三酯(TG)和总胆固醇(TC)水平为指标,观察ICAE对肥胖的预防作用;在实验第5周,连续5 d监测ICAE对小鼠24 h平均摄食量的影响;HE染色观察ICAE对脂肪组织的影响。分离和培养大鼠附睾来源的前脂肪细胞,尼罗红染色观察ICAE对分化脂肪细胞内脂滴形态的影响,Western blot法检测ICAE对细胞内过氧化物酶体增殖物激活受体γ(PPARγ)、脂滴包被蛋白1(Plin1)及激素敏感性脂肪酶(HSL)蛋白表达的影响。结果:ICAE可以显著抑制HFD引起的小鼠体重、腹内和皮下脂肪含量以及肝重的增加(P<0.01),并且显著降低血清TC和TG水平(P<0.01),但是对摄食量无显著影响;HE染色显示,ICAE能够显著减少HFD引起的白色脂肪细胞肥大。此外,在原代培养的大鼠分化脂肪细胞中,ICAE可以显著抑制细胞内脂滴的积累,并且显著下调脂肪分化关键转录因子PPARγ以及脂肪分化标志物Plin1和HSL的蛋白水平(P<0.01)。结论:ICAE对HFD诱导的小鼠肥胖具有预防作用,但对摄食量无明显影响;ICAE可以显著抑制脂肪分化过程,此作用可能与下调PPARγ的蛋白表达有关。AIM:To investigate the preventive effect of Ilex cornuta aqueous extract(ICAE)on high-fat diet(HFD)-induced obesity in mice and its effects on adipose differentiation.METHODS:Male Kunming mice were fed with HFD to induce obesity,ICAE were intragastrically administered at the same time,and orlistat was used as a positive control drug.The mice(n=39)were divided into 4 groups,including control group(n=10),obesity model group(n=9),orlistat treatment group(n=10),and ICAE treatment group(n=10).The body weight,the weight of intra-abdominal and subcutaneous adipose tissue,the liver weight,and the serum levels of triglyceride(TG)and total cholesterol(TC)were determined to evaluate the preventive effect of ICAE on obesity.Average 24-h food consumption of the mice were recorded for 5 times in the fifth week of the experiment to investigate the effect of ICAE on food intake.HE staining was used to observe the influence of ICAE on adipose tissue.The preadipocytes derived from rat epididymal fat pads were adopted to study the effect of ICAE on adipose differentiation.The morphological changes of lipid droplets were observed by Nile red staining,and the protein levels of peroxisome proliferator-activated receptor γ(PPARγ),perilipin 1(Plin1)and hormone-sensitive lipase(HSL) were examined by Western blot.RESULTS:ICAE significantly prevented the increases in the body weight,intra-abdominal and subcutaneous fat tissue weight,and the liver weight induced by HFD(P<0.01).ICAE also reduced the serum levels of TC and TG(P<0.01),but no effect on food intake was observed.Furthermore,HE staining showed that ICAE obviously prevented the enlargement of adipocytes induced by HFD.In addition,ICAE effectively inhibited the lipid droplet accumulation during differentiation,and significantly decreased the protein levels of PPARγ,a key adipogenic transcription factor,as well as the adipose differentiation markers Plin1 and HSL in primary rat differentiated adipocytes(P<0.01).CONCLUSION:ICAE has preventive effect on HFD-induced obesity,and has n

关 键 词：枸骨叶水提物 肥胖 脂肪分化 过氧化物酶体增殖物激活受体Γ 脂滴包被蛋白1 激素敏感性脂肪酶 

分 类 号：R965[医药卫生—药理学] R589.2[医药卫生—药学]