沉默TUG1基因对肝门部胆管癌QBC939细胞增殖、凋亡及周期的影响  

Effects of silencing TUG1 on the proliferation,apoptosis and cell cycle of QBC939 cells

作　　者：车宇 梁静 杨怡萍 廖娟 王蒨 蔡英全 邵帅 CHE Yu;LIANG Jing;YANG Yiping(Department of Radiotherapy,Shaanxi Cancer Hospital,Xi'an 710061)

机构地区：[1]陕西省肿瘤医院放疗科,西安710061

出　　处：《陕西医学杂志》2020年第5期538-541,591,共5页Shaanxi Medical Journal

摘　　要：目的:研究长链非编码RNA牛磺酸上调基因1(TUG1)对肝门部胆管癌QBC939细胞增殖、凋亡及周期的影响。方法:通过荧光实时定量PCR(RT-QPCR)检测人肝内胆管上皮细胞系HIBEpic、人肝门部胆管癌细胞系QBC939中TUG1的表达;通过RNA干涉技术沉默QBC939细胞中TUG1的表达,检测siRNA在QBC939细胞中对TUG1-siRNA的沉默效果;MTT法检测沉默TUG1对QBC939细胞增殖的影响;流式细胞术检测沉默TUG1对QBC939细胞凋亡和周期的影响。结果:肝门部胆管癌细胞QBC939中TUG1的表达较人肝内胆管上皮细胞HIBEpic显著上调;TUG1-siRNA可有效沉默QBC939细胞中TUG1的表达;沉默TUG1的表达可抑制QBC939细胞的增殖能力,促进QBC939细胞的细胞凋亡,使QBC939细胞周期阻滞于G1期。结论:TUG1在肝门部胆管癌细胞中表达上调并促进肿瘤细胞增殖,是潜在的治疗靶点。Objective:To investigate the effects of LncRNA TUG1 on the proliferation,apoptosis and cell cycle of human hilar cholangiocarcinoma line QBC939.Methods:TUG1 expression in human intrahepatic cholangiocarcinoma cell line HIBEpic and QBC939 were detected by quantitative real time PCR(RT-QPCR).The expression of TUG1 in QBC939 cells was silenced by RNA interference technology,and the silencing effect of siRNA was detected.MTT method was used to detect the effect of silence TUG1 on the proliferation of QBC939 cells.Flow cytometry was used to detect the effect of TUG1 on the apoptosis and cycle of QBC939 cells.Results:Compared with HIBEpic,the expression level of TUG1 in QBC939 cells was significantly higher,which was effectively silenced by TUG1-siRNA.Silencing the expression of TUG1 could inhibit the proliferation of QBC939 cells,promote the apoptosis of QBC939 cells,and block the cell cycle of QBC939 cells in G1 phase.Conclusion:The expression of TUG1 in hilar cholangiocarcinoma cells is up-regulated and promotes tumor cell proliferation,which is a potential therapeutic target.

关 键 词：长链非编码RNA 牛磺酸上调基因1 QBC939细胞 增殖 凋亡 细胞周期 

分 类 号：R735.8[医药卫生—肿瘤]