腺病毒介导的Hes1基因抑制肝细胞癌细胞的增殖与迁移  

Adenovirus-mediated Hes1 Expression Inhibits Proliferation and Migration of Hepatocellular Carcinoma Cells

作　　者：于兵[1] 尹刚[1] 胡以平[1] YU Bing;YIN Gang;HU Yiping(Department of Cell Biology,Navy Medical University(Second Military Medical University),Shanghai 200433,China)

机构地区：[1]海军军医大学(原第二军医大学)细胞生物学教研室,上海200433

出　　处：《肿瘤防治研究》2020年第3期164-169,共6页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(31771511,31401166)。

摘　　要：目的探讨Hes1基因对肝细胞癌细胞系Hep1-6细胞增殖、迁移与侵袭的影响。方法通过重组腺病毒载体介导Hes1基因在Hep1-6细胞系中过表达,随后测定细胞克隆形成率、增殖速率及体外迁移与侵袭能力的改变。结果Hep1-6细胞分别感染Ad-Hes1和阴性对照Ad-GFP后,感染Ad-Hes1的细胞系克隆形成数显著少于对照组(P<0.001);感染病毒6天后,CCK-8检测感染Ad-Hes1的细胞系在OD450 nm的吸光度值显著低于对照组(P<0.01);感染Ad-Hes1的细胞系24 h和48 h的划痕愈合率显著低于对照组(P<0.001);感染Ad-Hes1的细胞系在Transwell小室培养48 h后迁移进入Transwell下室的细胞数量显著低于对照组(P<0.001);感染Ad-Hes1的细胞系在铺有Matrigel基质胶的Transwell小室培养48 h后侵袭进入Transwell下室的细胞数量显著低于对照组(P<0.01)。结论Hes1有抑制Hep1-6细胞增殖、迁移与侵袭的作用。Objective To investigate the effects of Hes1 gene on the proliferation,migration and invasion of hepatocellular carcinoma cell line Hep1-6.Methods Cell proliferation,cell colony formation,migration and invasion were measured after Hes1 gene were overexpressed in Hep1-6 cells mediated by recombinant adenovirus.Results The clone numbers of Hep1-6 cells infected with Ad-Hes1 were significantly less than the control group infected with Ad-GFP(P<0.001).Six days after Ad-Hes1 infection,OD450nm of Hep1-6 cells were significantly lower than that of control group(P<0.01).The wound healing rates of Hep1-6 cells infected with Ad-Hes1 at 24h and 48h were significantly lower than those of the control group(P<0.001).The number of Hep1-6 cells infected with Ad-Hes1 migrating into the lower chamber of Transwell after 48 h of culture was significantly lower than that of the control group(P<0.001).The number of Hep1-6 cells infected with Ad-Hes1 invading into the lower chamber of Transwell after 48h of culture was significantly lower than that in the control group(P<0.01).Conclusion Hes1 could suppress the proliferation,migration and invasion of Hep1-6 cells.

关 键 词：HES1 腺病毒载体 肝细胞癌 增殖 迁移 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]