基于共轭聚合物的纳米粒子用于肿瘤的近红外二区荧光成像及光热治疗  

Conjugated-polymer Nanoparticle for NIR-II Fluorescence Imaging Guiding NIR-Ⅱ Photothermal Therapy

作　　者：黄婷[1] 陈妍[1] 孙鹏飞[1] 范曲立[1] 黄维[1] Ting Huang;Yan Chen;Peng-fei Sun;Qu-li Fan;Wei Huang(Key Laboratory for Organic Electronics&Information Displays,Institute of Advanced Materials,Nanjing University of Posts and Telecommunications,Nanjing 210023)

机构地区：[1]南京邮电大学有机电子与信息显示国家重点实验室培育基地信息材料与纳米技术研究院,南京210023

出　　处：《高分子学报》2020年第4期346-354,I0002,共10页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号21674042,21604048,21574064)资助项目.

摘　　要：为提高生物组织荧光成像质量以及对肿瘤的高效光热治疗,设计合成了一种新型的窄带隙共轭聚合物(BDT-TTQ),并通过纳米沉积的方式将聚合物制备成水溶性纳米粒子(BDT-TTQ NPs).该共轭聚合物纳米粒子在1000~1200 nm近红外二区范围具有较好的吸收,在1064 nm的激发光下能实现1200~1400 nm的近红外二区荧光成像. BDT-TTQ NPs纳米粒子粒径分布较窄,形貌呈规则的球形且分散均匀,具有好的生物相容性.该纳米粒子既可以在体外实现较高的近红外二区荧光成像穿透深度,又可以实现对小鼠活体血管的高清晰度的近红外二区荧光成像.此外,BDT-TTQ NPs纳米粒子在1064 nm激光下展现出优异的光热转换效率,具有较高的光毒性,对体外的肿瘤细胞以及小鼠的异质瘤具有高的光热杀伤能力.To improve the quality of fluorescence imaging and effectiveness of photothermal therapy, We designed a novel conjugated-polymer(BDT-TTQ) with a narrow band gap for NIR-Ⅱ fluorescence and NIR-Ⅱ photothermal effect. To realize solubility of BDT-TTQ in water, we enveloped the hydrophobic polymer BDTTTQ into amphiphilic copolymer(PEG-b-PPG-b-PEG, F-127) shells for NIR-Ⅱ water-soluble nanoparticles(BDT-TTQ NPs) through the nanoprecipitation method. With strong absorption in the NIR-Ⅱ region of 1000-1200 nm, the BDT-TTQ NPs can realize fluorescence image during 1200-1400 nm excited by 1064 nm laser.The prepared BDT-TTQ NPs were characterized by dynamic light scattering(DLS) and transmission electron microscopy(TEM). The average hydrodynamic radius of BDT-TTQ NPs was around 62 nm and spherical morphology was observed from TEM. Besides, the BDT-TTQ NPs showed the similar hydrodynamic radius in physiological environment such as phosphate buffer saline(PBS), Dulbecco’s Modified Eagle Medium(DMEM)and fetal bovine serum(FBS) and exhibited the excellent biological stability, indicating the potential for further in vivo application. To study the NIR-Ⅱ fluorescence characters, we firstly detected the maximum imaging depth of BDT-TTQ NPs in vitro and the penetration depth can achieve 6 mm at 1064 nm laser. High-resolution NIR-Ⅱ fluorescence imaging of living blood vessels in mice was also achieved by BDT-TTO NPs under 1064 nm laser irradiation. In addition, the real-time NIR-Ⅱ images of brain and abdomen were obtained with an ultrahigh signalto-background ratio. Photothermal experiments suggested the BDT-TTQ NPs exhibited excellent photothermal conversion and outstanding photothermla stability under 1064 nm excitation, which revealed the potential of BDTTTQ NPs for photothermal therapy in vivo. MTT assay and confocal laser scanning microscopy(CLSM) were used to analyse photothermal treatment toward human cervical carcinoma(HeLa) cells in vitro and the results indicated the nanoparticles performed an effective ph

关 键 词：共轭聚合物 1064 nm激发 近红外二区荧光成像 近红外二区光热 

分 类 号：R73-3[医药卫生—肿瘤] O631[医药卫生—临床医学] TB383.1[理学—高分子化学]