隐丹参酮上调GRP78抑制谷氨酸诱导的PC12细胞凋亡  

Inhibition of CTS on glutamate-induced PC12 apoptosis via GRP78 upregulation

作　　者：卢立敏 朱丽君 王守卫 金晓铃 邵晓轶 LU Limin;ZHU Lijun;WANG Shouwei;JIN Xiaoling;SHAO Xiaoyi(Department of Immunology,Medical School,Nantong University,Nantong 226001)

机构地区：[1]南通大学医学院免疫学系,南通226001

出　　处：《南通大学学报（医学版）》2020年第2期105-108,共4页Journal of Nantong University(Medical sciences)

基　　金：江苏省研究生科研与实践创新计划项目(KYCX18_2402),国家自然科学基金资助项目(81801580,81771767)。

摘　　要：目的:探讨隐丹参酮(Cryptotanshinone,CTS)对谷氨酸(glutamate,Glu)诱导的神经元凋亡的影响及潜在的作用机制。方法:取生长状态良好的PC12细胞,用1μmol/L Glu处理,Western Blot检测细胞中凋亡相关蛋白Bax、Bcl2的表达水平;以CTS(5μmol/L或10μmol/L)预处理PC12细胞12 h后,再用Glu刺激12 h,采用TUNEL染色分析CTS对Glu诱导PC12细胞凋亡的影响;Western Blot检测CTS预处理前后Glu刺激的PC12细胞中糖蛋白调节78(78 ku glucose-regulated protein,GRP78)的蛋白表达水平,并通过sh-RNA干扰细胞内GRP78表达,观察细胞活力的变化。结果:Glu可诱导PC12细胞凋亡,抑制胞内GRP78的表达;经CTS预处理后Glu诱导的神经元凋亡现象得到缓解,GRP78表达增加;干扰PC12细胞内GRP78表达后,可逆转CTS的抗凋亡作用。结论:CTS可通过上调GRP78的表达从而保护PC12细胞抵抗Glu诱导的细胞凋亡。Objective:To investigate the effect of cryptotanshinone(CTS)on glutamate(Glu)induced neuron apoptosis and explore the potential mechanisms.Methods:PC12 cells with good growth status were treated with Glu(1μmol/L),and the protein levels of Bax and Bcl2 were detected by Western Blot.PC12 cells were then stimulated with Glu before they were pretreated with CTS(5μmol/L or 10μmol/L)for 12 hours.The effect of CTS on Glu-induced PC12 cell apoptosis was analyzed by TUNEL staining,and Western Blot was used to detected 78 ku glucose-regulated protein(GRP78)expression;knocking down the expression of GRP78 by RNA interference technology to observe the changes of cell viability.Results:Glu could induce the apoptosis of PC12 cells and inhibit the expression of GRP78.After CTS pretreatment,glutamated-induced neuron apoptosis was alleviated and GRP78 expression was increased;interferenced with GRP78 expression in PC12 cells could reverse the anti-apoptotic effects of CTS.Conclusion:CTS can protect PC12 cells from glutamate-induced apoptosis by up-regulating the expression of GRP78.

关 键 词：隐丹参酮 谷氨酸 糖调节蛋白78 PC12细胞凋亡 

分 类 号：R363[医药卫生—病理学]