肾素基因启动子区域甲基化水平与精神分裂症患者认知损害的关联  

The association between methylation level of renin gene promoter and the cognitive impairment of schizophrenia patients

作　　者：谷晓楚[1,2] 倪培艳[1,3,4] 李涛[1,3,4] GU Xiaochu;NI Peiyan;LI Tao(Psychiatric Laboratory,West China Hospital of Sichuan University,Cheng-du 610041;Clinical Laboratory,the Affiliated Guangji Hospital of Soochow University;Mental Health Center;Huaxi Brain Research Center,West China Hospital of Sichuan University)

机构地区：[1]四川大学华西医院精神医学研究室,成都610041 [2]苏州大学附属广济医院临床检验科 [3]四川大学华西医院心理卫生中心 [4]四川大学华西医院脑科学研究中心

出　　处：《南通大学学报（医学版）》2020年第2期117-121,共5页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金面上项目(81871054);国家自然科学基金青年基金资助项目(81501159);高等学校博士学科点专项科研基金资助项目(20130181120032);苏州市产业技术创新专项(民生科技-医疗卫生应用基础研究)(SYSD2016141)。

摘　　要：目的:探索肾素基因启动子甲基化水平与精神分裂症可能的相关性及潜在的生物学作用。方法:利用外周血单个核细胞基因组,采用Massarray飞行时间质谱法,测定肾素基因启动子CpG位点的甲基化水平。采用剑桥神经心理自动化成套测试中的快速视觉信息处理(rapid visual information processing,RVP)和延迟匹配(delayed matching to sample,DMS)评估受试者注意力及视觉记忆。结果:发现两组间肾素基因启动子甲基化水平差异无统计学意义(P>0.05)。以性别、年龄、患病状态作协变量,CpG位点[chr1:204166158(hg38)]的甲基化比例与RVP总正确反应数(r=-0.68,P=2.42×10^-7),RVP A’(r=-0.61,P=6.94×10^-6),DMS总正确数(r=-0.55,P=1.02×10^-4),DMS延迟正确数(r=-0.54,P=1.52×10^-4)显著相关。结论:肾素基因可能通过启动子甲基化在精神分裂症病理中发挥一定的作用。Objective:To explore the potential correlation and biological role of renin gene promoter methylation on patho-genesis of schizophrenia.Methods:The methylation level of CpG sites in the promoter regin from peripheral blood mononuclear cell of schizophrenia patients and healthy controls were examined using Mass Array time-of-flight mass spectrometry system.The rapid visual information processing(RVP)and delayed matching to samples(DMS)tests of Cambridge neuropsychological test automatic battery were performed to assess the attention and visual memory of subjects form two groups.Results:The methylation levels of all CpG in renin gene promoter showed no sig-nificant difference between two groups(P>0.05).After setting gender,age and disease status as covariate,the methylation level of CpG[chr1:204166158(hg38)]was significantly correlated with RVP total correct rejection(r=-0.68,P=2.42×10^-7),RVP A’(r=-0.61,P=6.94×10^-6),DMS total correct(r=-0.55,P=1.02×10^-4),DMS total correct all delays(r=-0.54,P=1.52×10^-4).Conclusion:The renin gene may play a certain role in the pathology of schizophrenia through promoter methylation.

关 键 词：精神分裂症 肾素 DNA甲基化 基因启动子 认知功能 

分 类 号：R749.3[医药卫生—神经病学与精神病学]