miR-506和FGF2在上皮性卵巢癌中的表达及临床意义  

Expression and clinical significance of miR-506 and FGF2 in epithelial ovarian cancer

作　　者：张玉苓 李元昆[1] 李幸丽 Zhang Yuling;Li Yuankun;Li Xingli(Second Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450014,China;Pingmei Shenma Medical Group General Hospital,Henan Pingdingshan 467012,China)

机构地区：[1]郑州大学第二附属医院,河南郑州450014 [2]平煤神马医疗集团总医院,河南平顶山467012

出　　处：《现代肿瘤医学》2020年第12期2132-2137,共6页Journal of Modern Oncology

摘　　要：目的:检测上皮性卵巢癌组织微小RNA-506(miR-506)和成纤维细胞生长因子2(FGF2)表达情况,并探究其临床意义。方法:选取2011年3月至2012年12月本院收治的上皮性卵巢癌患者56例,选择同期因子宫良性病变在平煤神马医疗集团总医院接受全子宫双附件切除的患者56例(对照组);采用实时定量PCR(qRT-PCR)法检测miR-506表达水平,采用免疫组化法检测FGF2表达水平,采用全自动化学发光免疫分析仪检测血清糖类抗原125(CA125)水平。结果:上皮性卵巢癌组织miR-506表达水平较正常卵巢组织显著降低(P<0.05),FGF2表达量显著高于正常卵巢组织(P<0.05),FGF2蛋白表达阳性率较正常卵巢组织显著升高;上皮性卵巢癌组织中miR-506、FGF2表达与患者临床分期、有无淋巴结转移、血清CA125水平有关(P<0.05),与患者年龄、组织学类型无关(P>0.05);miR-506高表达上皮性卵巢癌患者术后5年总生存率显著高于低表达者(P<0.05),FGF2高表达上皮性卵巢癌患者术后5年总生存率显著低于低表达患者(P<0.05);Pearson检验结果显示,卵巢癌肿瘤组织miR-506与FGF2表达水平负相关。结论:miR-506在上皮性卵巢癌组织中低表达,FGF2高表达,与患者临床分期、淋巴结转移、血清CA125水平有关,可能作为上皮性卵巢癌患者病情监测及预后的生物指标。Objective:To investigate the expressions of microRNA-506(miR-506)and fibroblast growth factor 2(FGF2)in ovarian cancer tissues and their clinical significances.Methods:From March 2011 to December 2012,56 patients with ovarian cancer were selected.And 56 patients with benign lesions of the same period were enrolled in this hospital(control group).Real time quantitative PCR(qRT-PCR)was used to detect the expression level of miR-506.Immunohistochemistry was used to detect the expression level of FGF2 protein.The level of serum carbohydrate antigen 125(CA125)was detected by ELISA.Results:The expression level of miR-506 in ovarian cancer tissues was significantly lower than that in control tissues(P<0.05).The expression level of FGF2 in ovarian cancer tissues was significantly higher than that in normal tissues.The positive rate of FGF2 expression was significantly higher than that in normal tissues.The expression of miR-506 and FGF2 in ovarian cancer tissues was significantly correlated with clinical stage,lymph node metastasis and serum CA125 level(P<0.05),and not correlated with age and histological type(P>0.05).The postoperative 5-year overall survival rate of patients with miR-506 high expression was significantly higher than that of patients with low expression(P<0.05).The postoperative 5-year overall survival rate of patients with FGF2 high expression was significantly lower than that of patients with low expression(P<0.05).The levels of miR-506 and FGF2 in ovarian cancer tissues were negatively correlated(P<0.05).Conclusion:The expression of miR-506 in ovarian cancer tissues is decreased significantly,and the expression of FGF2 is increased significantly.They may be related to clinical stage,lymph node metastasis and serum CA125 level of ovarian cancer.They may be used as biological indicators for monitoring and prognosis of ovarian cancer patients.

关 键 词：miR-506 成纤维细胞生长因子2 上皮性卵巢癌 预后 

分 类 号：R737.31[医药卫生—肿瘤]