19号染色体开放阅读框5调节自噬活性与结直肠癌恶性程度和紫杉醇化疗敏感性的关系  

Relationship between autophagy regulated by chromosome 19 open reading frame 5 and malignant degree of colorectal cancer and chemosensitivity of paclitaxe

作　　者：闫炳文 李胜龙[1] 王春敬[1] 乔鹏飞[1] 王夫景[1] Yan Bingwen;Li Shenglong;Wang Chunjing;Qiao Pengfei;Wang Fujing(Department of General Surgery,the Second Affiliated Hospital of Harbin Medical University,Harbin 150086,China)

机构地区：[1]哈尔滨医科大学附属第二医院普外十科,150086

出　　处：《中国医师进修杂志》2020年第5期439-443,共5页Chinese Journal of Postgraduates of Medicine

基　　金：哈尔滨市科技攻关计划(2017AB9BS038)。

摘　　要：目的观察19号染色体开放阅读框5(C19ORF5)调节自噬活性与结直肠癌恶性程度和紫杉醇化疗敏感性的关系。方法选取哈尔滨医科大学附属第二医院2015—2017年141例结直肠癌患者的肿瘤组织和癌旁正常组织,采用免疫组织化学法和实时定量聚合酶链反应法检测C19ORF5蛋白和mRNA的表达情况,探讨C19ORF5蛋白调节自噬活性与结直肠癌恶性程度的相关性。141例患者均行术后化疗,91例采用常规化疗方案(卡培他滨联合奥沙利铂,常规化疗组),50例采用常规方案联合紫杉醇化疗(紫杉醇组),两组均治疗6个疗程。结果癌组织在透射电镜下可见自噬体,C19ORF5蛋白为淡黄色至棕褐色颗粒,表达于细胞质;癌组织C19ORF5蛋白染色强度明显强于正常组织,且Ⅱ期染色强度明显高于Ⅳ期。Ⅰ~Ⅱ期患者C19ORF5蛋白高表达率明显高于Ⅲ~Ⅳ期患者[83.3%(25/30)比17.1%(19/111)],差异有统计学意义(P<0.01)。癌组织C19ORF5 mRNA表达水平明显高于正常组织(1.17±0.45比0.82±0.29),差异有统计学意义(P<0.05)。癌组织C19ORF5蛋白表达水平与肿瘤分期、癌胚抗原和肝转移有关(P<0.01或<0.05),癌组织C19ORF5蛋白表达水平与淋巴结转移无关(P>0.05)。常规化疗组91例患者中,有效70例(76.9%),无效21例(23.1%);紫杉醇组50例患者中,有效42例(84.0%),无效8例(16.0%),两组有效率比较差异无统计学意义(P>0.05)。在常规化疗组中,有效患者和无效患者化疗前癌组织与化疗后血清C19ORF5蛋白表达水平比较差异均无统计学意义(P>0.05);有效患者与无效患者化疗后血清C19ORF5蛋白表达水平比较差异无统计学意义(P>0.05)。在紫杉醇组中,有效患者化疗前癌组织C19ORF5蛋白表达水平明显高于化疗后血清C19ORF5蛋白表达水平(0.9±0.3比0.5±0.2),有效患者化疗后血清C19ORF5蛋白表达水平明显低于无效患者(0.5±0.2比0.8±0.2),差异有统计学意义(P<0.05)。结论C19ORF5蛋白的高表达可以提高�Objective To observe the relationship between autophagy regulation activity of chromosome 19 open reading frame 5(C19ORF5)and malignant degree of colorectal cancer and chemosensitivity of paclitaxel.Methods The tumor tissues and normal adjacent tissues of 141 colorectal cancer patients from 2015 to 2017 in the Second Affiliated Hospital of Harbin Medical University were selected.The expressions of C19ORF5 protein and mRNA were detected by immunohistochemistry and timed quantitative polymerase chain reaction.The correlation between C19ORF5 protein regulating autophagy activity and malignancy of colorectal cancer was analyzed.All 141 patients received postoperative chemotherapy,among whom 91 patients received conventional chemotherapy(capecitabine combined with oxaliplatin,conventional chemotherapy group),and 50 patients received conventional chemotherapy combined with paclitaxel(paclitaxel group).Six course of treatment was treated in both groups.Results Autophagosomes could been seen under transmission electron microscopy.C19ORF5 protein was pale yellow to tan granules,and was expressed in the cytoplasm.The C19ORF5 protein staining intensity of cancer tissue was significantly stronger than that of normal tissue,and the staining intensity of stageⅡwas significantly higher than that of stageⅣ.The high expression rate of C19ORF5 protein in patients with stageⅠtoⅡwas significantly higher than that in patients with stageⅢtoⅣ:83.3%(25/30)vs.17.1%(19/111),and there was statistical difference(P<0.01).The expression of C19ORF5 mRNA in cancer tissues was significantly higher than that in normal tissues:1.17±0.45 vs.0.82±0.29,and there was statistical difference(P<0.05).The expression level of C19ORF5 protein in cancer tissue was related to tumor stage,carcinoembryonic antigen and liver metastasis(P<0.01 or<0.05);the expression level of C19ORF5 protein in cancer tissue was not related to lymph node metastasis(P>0.05).Of the 91 cases in conventional chemotherapy group,chemotherapy was effective in 70 cases(76.9

关 键 词：结直肠肿瘤 自噬 药物疗法 19号染色体开放阅读框5 紫杉醇 

分 类 号：R735[医药卫生—肿瘤]