基于X线同步辐射吸收CT和X线相衬CT牙周炎大鼠模型离体上颌骨成像  

Imaging of vitro maxilla in rat periodontitis models based on X-ray absorption-contrast CT and X-ray phase-contrast CT

作　　者：杨婷婷 赵雨晴 吕文娟 刘大勇[2] 辛晓红 胡春红 YANG Tingting;ZHAO Yuqing;LYU Wenjuan;LIU Dayong;XIN Xiaohong;HU Chunhong(College of Biomedical Engineering and Technology,Tianjin Medical University,Tianjin 300070,China;Department of Endodontcs,Tianjin Medical University School of Stomatology,Tianjin 300070,China)

机构地区：[1]天津医科大学生物医学工程与技术学院,天津300070 [2]天津医科大学口腔医学院牙体牙髓科,天津300070

出　　处：《中国医学影像技术》2020年第5期653-658,共6页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金项目(81671683);天津市自然科学基金项目(16JCYBJC28600)。

摘　　要：目的比较大鼠离体上颌骨X线同步辐射吸收CT(ACCT)和X线相衬CT(PCCT)成像特点,观察PCCT技术评估牙周炎大鼠模型的价值。方法将6只雄性SD大鼠随机分为牙周炎组(n=3)和正常组(n=3);对牙周炎组采用“丝线结扎+高糖饲养”法构建模型,并以10%高糖黏性饲料喂养8周,正常组予常规饲养。8周后取2组大鼠右上颌骨行ACCT和PCCT,分析其特点,并重建上颌骨3D图像,测量并比较2组大鼠釉牙骨质界到牙槽嵴顶的距离(CEJ-ABC)。结果ACCT图像无法清晰完整显示牙髓,难以分辨牙周膜软组织;PCCT图像组织对比度佳,能清晰展现上颌骨软、硬组织的微观结构,且基于PCCT重构3D图像质量优于ACCT。相比正常组,牙周炎组CEJ-ABC明显增大(Z=-9.43,P<0.01)。结论利用PCCT进行大鼠上颌骨软硬组织成像较ACCT更具优势,对评估牙周炎大鼠模型上颌骨具有重要价值。Objective To compare imaging characteristics of rat vitro maxilla based on X-ray absorption-contrast CT(ACCT)and X-ray phase-contrast CT(PCCT),and to explore the value of PCCT in evaluating rat periodontitis models.Methods A total of 6 SD rats were randomly divided into periodontitis group and normal group(each n=3).In periodontitis group,"ligation of silk thread+high sugar feeding"method was used to establish periodontitis models,and the rats were given 10%high sugar viscous feed for 8 weeks,while rats in control group were given conventional feeding.After 8 weeks,ACCT and PCCT were performed on the right maxilla of rats in both groups,and imaging characteristics were analyzed.Then 3D images of the maxilla were reconstructed,and the distance between cemento-enamel junction and alveolar bone crest(CEJ-ABC)of rats were measured and compared between groups.Results ACCT could not clearly display dental pulp,also difficult to distinguish the periodontal membrane soft tissue.The contrast of PCCT images were better,able to clearly show the microstructure of soft tissue and hard tissue of maxilla,and the quality of 3D images based on PCCT was better than that of ACCT.Compared with control group,CEJ-ABC increased significantly in periodontitis group(Z=-9.43,P<0.01).Conclusion PCCT has more advantages in imaging of soft tissue and hard tissue of rat maxilla,which is of great value in assessment of maxilla in rat periodontitis models.

关 键 词：牙周炎 上颌骨 大鼠 X射线相衬成像 X线吸收衬度成像 

分 类 号：R-332[医药卫生] R814.42