肾精亏虚对SD雄性大鼠造血功能影响实验研究  

Effect of Kidney Essence Deficiency on Hematopoiesis in Male SD Rats

作　　者：吕建芳 周安方[1] 萧闵[1] LYU Jianfang;ZHOU Anfang;XIAO Min(Hubei University of Chinese Medicine,Wuhan 430065;Hubei University,Wuhan 430062)

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]湖北大学,湖北武汉430062

出　　处：《湖北中医药大学学报》2020年第1期5-8,共4页Journal of Hubei University of Chinese Medicine

基　　金：湖北省教育厅科学研究计划指导项目(项目编号:B2019098)。

摘　　要：目的观察肾精亏虚对SD雄性大鼠骨髓造血功能影响。方法将30只SD大鼠分为对照组、模型组、补肾组各10只,仿地震平台刺激+皮下注射苯+环磷酰胺腹腔注射复制肾精亏虚大鼠模型,补肾组给予龟鹿二仙胶灌胃,分别于第1天、第14天、第28天、第42天、第49天动态观察血红细胞(RBC)及血红蛋白(HGB)变化;ELISA法检测血清睾酮(T)、促血红细胞生成素(EPO)含量;全精子分析仪检测附睾精子质量;HE染色观察睾丸病理观察;制备骨髓涂片瑞氏染色检测骨髓单核细胞变化。结果与对照组比较,模型组、补肾组大鼠RBC、HGB显著性下降(P<0.05或P<0.01),血清T、EPO显著性下降(P<0.05或P<0.01);与对照组比较,模型组精子数量及活力均下降(P<0.05),睾丸生精细胞排列稀疏,骨髓单核细胞减少(P<0.05);与模型组比较,补肾组大鼠血常规RBC、HGB升高(P<0.05或P<0.01),血清T、EPO显著性升高(P<0.05),精子质量改善、睾丸生精细胞增多、骨髓单核细胞增加(P<0.05)。结论肾精亏虚对大鼠造血功能产生影响,为"肾藏精,精者,血之所成也"提供实验依据。Objective To observe the effects of kidney essence deficiency on hematopoiesis in male SD rats.Methods30 SD rats were divided into control group,model group and kidney tonifying group,10 rats each.Rat model of kidney essence deficiency induced by simulated earthquake stimulation+substaneous injection of Benzene and cyclophosphamide intraperitoneal injection.The changes of red blood cell(RBC)and hemoglobin(HGB)were observed at 1 d,14 d,28 d,42 d and 49 d after modeling respectively;serum testosterone(T)and erythropoietin(EPO)were detected by ELISA;epididymal sperm quality was detected by total sperm analyzer,the testicular pathology was observed by HE staining,and the mononuclear cells of bone marrow were detected by Rayleigh staining.Results Compared with control group,RBC,HGB,serum T and EPO in model group decreased significantly(P<0.01),the concentration and activity of sperm decreased significantly(P<0.05),the arrangement of spermatogenic cells in testis was sparse,and mononuclear cells in bone marrow decreased(P<0.01);compared with model group,RBC,HGB,serum T and EPO in kidney tonifying group increased significantly(P<0.05 or P<0.01);the quality of spermatozoa,spermatogenic cells of testis and mononuclear cells of bone marrow increased(P<0.05).Conclusion Deficiency of kidney essence has an effect on the hematopoietic function of rats,which provides the experimental basis for the theory of"the kidney stores essence,which builds blood".

关 键 词：肾精亏虚 骨髓 促红细胞生成素 

分 类 号：R229[医药卫生—中医基础理论]