miR-195-5p靶向调控ELMO2抑制IL-17诱导的胃癌细胞AGS侵袭和迁移  

miR-195-5p targeted regulation of ELMO2 inhibits invasion and migration of AGS cells induced by IL-17

作　　者：钟晨[1] 张丽翠[2] 朱美意[3] ZHONG Chen;ZHANG Li-Cui;ZHU Mei-Yi(Department of Third General Surgery,First Affiliated Hospital of Medical College of Shihezi University,Shihezi 832000,China)

机构地区：[1]石河子大学医学院第一附属医院普外三科,石河子832000 [2]石河子大学医学院第一附属医院检验科,石河子832000 [3]石河子大学医学院第一附属医院急诊科,石河子832000

出　　处：《中国免疫学杂志》2020年第9期1063-1068,共6页Chinese Journal of Immunology

摘　　要：目的:研究miR-195-5p调控吞噬细胞运动蛋白2(ELMO2)对白介素17(IL-17)诱导的胃癌细胞AGS侵袭和迁移的影响和机制。方法:以胃癌细胞AGS作为实验对象,转染miR-195-5p mimics,给予IL-17处理,qRT-PCR方法测定细胞中miR-195-5p表达;Transwell小室测定细胞侵袭和迁移能力;Western blot测定细胞中E-cadherin、Vimentin蛋白表达水平。生物信息学软件预测miR-195-5p的靶基因可能为ELMO2,双荧光素酶系统鉴定靶向关系。在胃癌细胞AGS中共转染miR-195-5p mimics、pcDNA3.1-ELMO2,检测细胞侵袭和迁移能力变化。结果:IL-17处理后的胃癌细胞AGS中miR-195-5p表达水平下调,细胞侵袭和迁移能力升高,细胞中E-cadherin蛋白表达水平下降,Vimentin蛋白表达水平升高。转染miR-195-5p mimics后的细胞经过IL-17处理以后,细胞中miR-195-5p表达水平升高,细胞侵袭和迁移能力降低,细胞中E-cadherin蛋白表达水平升高,Vimentin蛋白表达水平降低。miR-195-5p靶向负调控ELMO2表达。pcDNA3.1-ELMO2能够提高转染miR-195-5p mimics后的细胞侵袭和迁移能力。结论:miR-195-5p靶向负调控ELMO2抑制IL-17诱导的胃癌细胞AGS侵袭和迁移。Objective:To study the effect and mechanism of miR-195-5 p on the invasion and migration of AGS cells induced by IL-17.Methods:The gastric cancer cell line AGS was used as the experimental object,miR-195-5 p mimics was transfected,IL-17 was given,the expression of miR-195-5 p was detected by qRT-PCR;cell invasion and migration were measured by Transwell chamber.The expression levels of E-cadherin and vimentin were measured by Western blot.Bioinformatics software predicted that the target gene of miR-195-5 p might be ELMO2,target relationship was identified by double luciferase system.miR-195-5 p mimics and pcDNA3.1-ELMO2 were transfected into AGS cells,the ability of cell invasion and migration was detected.Results:The expression of miR-195-5 p was down regulated in AGS cells treated with IL-17,cell invasion and migration increased,the expression level of E-cadherin protein decreased,Vimentin protein expression level increased.The cells transfected with miR-195-5 p mimics were treated with IL-17,the expression level of miR-195-5 p was increased,the ability of cell invasion and migration was reduced,the expression level of E-cadherin protein was increased,Vimentin protein expression level decreased.miR-195-5 p targeted negative regulation of ELMO2 expression.pcDNA3.1-ELMO2 could improve the cell invasion and migration ability of miR-195-5 p mimics.Conclusion:miR-195-5 p targeted negative regulation of ELMO2 inhibited the invasion and migration of AGS cells induced by IL-17.

关 键 词：miR-195-5p 白介素17 胃癌 侵袭 吞噬细胞运动蛋白2 

分 类 号：R735.2[医药卫生—肿瘤]