248例宫颈上皮内瘤变患者人乳头瘤病毒感染状况分析  

HPV infection in 248 patients with cervical intraepithelial neoplasia

作　　者：季冰 郭芝亮 易莉莎 黄子健 龚亦华 JI Bing;GUO Zhiliang;YI Lisha;HUANG Zijian;GONG Yihua(Department of Oncology,Maternal and Infant Hospital of Guangzhou Women and Children's Medical Center,Guangzhou,Guangdong 510000,China)

机构地区：[1]广东省广州市妇女儿童医疗中心妇婴院区肿瘤妇科,广东广州510000

出　　处：《中国医学工程》2019年第8期50-53,共4页China Medical Engineering

摘　　要：目的研究和分析宫颈上皮内瘤变患者人乳头瘤病毒(HPV)感染状况。方法选取2016年至2017年在该院就诊以及治疗的248例病理诊断为宫颈组织病变患者采用聚合酶链式反应(PCR)扩增及导流杂交基因芯片技术对患者宫颈脱落细胞进行HPV基因分型检测,观察不同年龄阶段及不同级别宫颈上皮内瘤变患者HPV的感染状况。结果248份样本中,共234份检测出HPV阳性,阳性率为94.35%;其中单一基因型139份,占58.90%,多基因型混合感染78份,占33.05%,其他型占8.05%;根据宫颈上皮内瘤变(CIN)患者的年龄将其分组,结果显示31~40岁组HPV检出阳性率最高为33.47%,高于20~30岁组及41~50岁组,分别为22.46%及25.42%,明显高于51~60岁组的11.86%;各年龄组之间差异有统计学意义(χ^2=32.017,P<0.01)。CIN Ⅰ、CIN Ⅱ、宫颈原位癌组均以高危型HPV16感染率最高。HPV感染检出率在宫颈息肉和子宫平滑肌瘤组及CIN Ⅰ、CIN Ⅱ、宫颈原位癌组分别为0、75.00%、93.62%和79.07%,随着宫颈病变级别的增加,HPV的总阳性率逐渐上升(P<0.05)。结论正常宫颈及不同级别的CIN患者HPV感染情况不同,不同亚型HPV在不同程度的CIN患者中的感染率不同,不同年龄对HPV的感染率也有所不同。【Objective】To study and analyze the human papillomavirus(HPV)infection in 248 patients with cervical intraepithelial neoplasia(CIN).【Methods】A total of 248 patients with cervical lesions as histopathologically positive who were treated in our hospital from 2016 to 2017 were selected,and the HPV genotyping of cervical exfoliated cells were detected by polymerase chain reaction(PCR)amplification and flow-through hybridization and gene chip technology.Then,the infection status of HPV of patients with cervical intraepithelial neoplasia at different ages and grades were observed.【Results】Of 248 samples,234 cases were HPV positive,the positive rate was 94.35%;139 cases were single genotype,the proportion was 58.90% and 78 cases were mixed polygenotype,the proportion was 33.05% and 8.05% were other types.They were grouped according to the age of CIN patients,the results showed that the highest positive rate of HPV was 33.47% in 31-40 years old group,which was higher than 22.46%in 20-30 years old group and 25.42% in 41-50 years old group,and significantly higher than 11.86%in 51-60 years old group.There were significant differences among different age groups(χ^2=32.017,P<0.01).The high-risk HPV16 infection rate was the highest among the CIN Ⅰ,CIN Ⅱ,cervical carcinoma in situ.The detection rates of HPV infection in cervical polyps,leiomyoma,CIN Ⅰ,CIN Ⅱ and carcinoma in situ were 0,75.00%,93.62%and 79.07%respectively.With the increase of cervical lesion grade,the total positive rate of HPV was increased gradually(P<0.05).【Conclusion】The infection rates of HPV in normal cervix and CIN patients of different grades are different,different subtypes of HPV have different infection rates in different degrees of CIN patients,and the infection rate of HPV varies with age.

关 键 词：宫颈上皮内瘤变 人乳头瘤病毒 导流杂交基因芯片技术 基因型 感染率 

分 类 号：R737.33[医药卫生—肿瘤]