葡萄籽多酚提取条件优化及其脂肪酶抑制影响  

Parameters optimizing for extracting polyphenols in grape seed and the inhibition effect of extract on lipase activity

作　　者：姜帆 吴子健[1] 侯惠静 张一然 JIANG Fan;WU Zi-jian;HOU Hui-jing;ZHANG Yi-ran(College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin Key Laboratory of Food Biotechnology,Tianjin 300134,China;College of Food Engineering,Tianshi University,Tianjin 301700,China;College of Arts,Tianjin University of Commerce,Tianjin 300134,China)

机构地区：[1]天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津300134 [2]天津天狮学院食品工程学院,天津301700 [3]天津商业大学艺术学院,天津300134

出　　处：《甘肃农业大学学报》2020年第2期148-156,共9页Journal of Gansu Agricultural University

基　　金：天津市高等学校科技发展基金计划项目(20130611);天津市应用基础与前沿技术研究计划青年项目(18JCQNJC84600).

摘　　要：【目的】研究葡萄籽中多酚的最优提取工艺条件并测定其对脂肪酶的抑制率.【方法】通过响应面法优化葡萄籽多酚的提取工艺,然后分析不同体积分数乙醇对AB-8大孔吸附树脂纯化的葡萄籽多酚的脂肪酶抑制率.【结果】葡萄籽多酚最佳提取条件为70%乙醇、纤维素酶添加量为0.5 mg/mL、料液比1∶10、提取温度54℃、提取时间39 min、提取次数4次;得到的葡萄籽提取物对胰脂肪酶活性抑制活力IC50值为2.46 mg/mL.【结论】经过条件优化,多酚的提取率提高,所提取的多酚具有胰脂肪酶活性抑制活力.AB-8大孔吸附树脂法中不同乙醇洗脱组分对脂肪酶活力的抑制作用有一定差异,其中40%乙醇洗脱的葡萄籽多酚溶液对脂肪酶抑制效果最好.【Objective】 To optimize the conditions for extracting the polyphenols in grape seed and determine the lipase inhibition rate of extracted polyphenols.【Method】 The optimal process was obtained with response surface methodology.And the lipase inhibition rate of extracted polyphenols purified with AB-8 macroporous adsorption chromatography and eluted by ethanol solutions of different concentration was analyzed.【Result】 The results showed that the optimal extraction conditions were 70% ethanol,and the added amount of cellulase was 0.5 mg/mL,1∶10 solid-liquid ratio,extraction temperature 54 ℃,extraction time 39 min,extraction times 4 times.The IC50 value of lipase activity inhibition activity was 2.46 mg/mL.【Conclusion】 After the optimization,the extraction rate of polyphenols is increased,and the extracted polyphenols show inhibition activity on pancreatic lipase activity.In treatments of the AB-8 macroporous adsorption resin method,the inhibitory effects of different ethanol-eluting components on lipase activity are different,and 40% ethanol-eluted grape seed polyphenol solution has the best inhibitory effect on lipase.

关 键 词：葡萄籽多酚提取物 脂肪酶抑制活性 响应面法 大孔吸附层析 

分 类 号：TS209[轻工技术与工程—食品科学] R284.2[轻工技术与工程—食品科学与工程]