外加电压对污泥厌氧消化产甲烷同步降解菲的影响  

Effects of different voltages on methane production andphenanthrene degradation in sludge anaerobic digestion

作　　者：林超霸 李莲 祝佩茹 费吉东 谢欣欣[1,2] 贾红华[1,2] 雍晓雨[1,2] 吴夏芫[1,2] 周俊[1,2] LIN Chaoba;LI Lian;ZHU Peiru;FEI Jidong;XIE Xinxin;JIA Honghua;YONG Xiaoyu;WU Xiayuan;ZHOU Jun(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China;Bioenergy Research Institute,Nanjing Tech University,Nanjing 211800,China;College of Environment,Nanjing Tech University,Nanjing 211800,China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800 [2]南京工业大学生物能源所,江苏南京211800 [3]南京工业大学环境学院,江苏南京211800

出　　处：《生物加工过程》2020年第3期303-311,共9页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21777069);国家重点研发计划(2016YFE0112800);江苏省先进生物制造创新中心自主研发课题(XTE1832);江苏省“青蓝工程”;江苏省“六大人才”高峰。

摘　　要：在污泥厌氧消化与微生物电解池耦合系统中,研究不同外加电压对污泥产甲烷及降解菲的影响,外加电压分别设定为0.4、0.8、1.2和2.5 V,定期对消化过程中产气量、pH、总有机碳(TOC)、溶解性化学需氧量(SCOD)、氨氮及菲含量等参数进行分析,并通过高通量测序对消化污泥及电极生物膜上的微生物多样性进行分析。结果表明:外加电压能有效促进污泥厌氧消化产甲烷并提高系统中菲的降解率,当外加电压为0.8 V时,单位质量固体的甲烷产率为136.36 L/kg,挥发性固体(VS)的去除率为42.26%,污染物菲的去除率为43.88%,均明显高于其他实验组。微生物多样性分析表明:当外加电压低于0.8 V时,阴极生物膜上的优势产甲烷菌为Methanosaeta和Methanospirillum,属于乙酸营养性产甲烷菌;当外加电压为0.8 V以上时,阴极生物膜上的优势产甲烷菌为Methanobacterium,属于氢营养性产甲烷菌。In a coupled system of sludge anaerobic digestion and microbial electrolysis cell,the effects of different voltages on methane production and phenanthrene degradation were studied.The applied voltages were set to 0.4,0.8,1.2,and 2.5 V.The biogas production,pH,total organic carbon(TOC),soluble chemical oxygen demand(SCOD),ammonia nitrogen,and phenanthrene content were measured during the digestion process,and the microbial diversity on the digestive sludge and electrode biofilm was analyzed by high-throughput sequencing.The results showed that the applied voltage could effectively promote the anaerobic digestion of sludge to produce methane and improve the degradation rate of phenanthrene in the system.When the applied voltage was 0.8 V,the methane yield per solid was 136.36 L/kg.The removal rate of volatile solid(VS)was 42.26%,and the removal rate of pollutant phenanthrene was 43.88%,which was significantly higher than others.Microbial diversity analysis showed that when the applied voltage was lower than 0.8 V,the dominant methanogens on the cathode biofilm were Methanosaeta and Methanospirillum,which belonged to the acetoclastic methanogen.When the applied voltage was above 0.8 V,the dominant methanogen on the cathode biofilm was Methanobacterium,which was hydrogenotrophic methanogen.

关 键 词：污泥厌氧消化 微生物电解池 菲 微生物多样性 产甲烷菌 

分 类 号：X705[环境科学与工程—环境工程]