一株Agrobacterium sp.异养硝化菌的分离鉴定及其氨氧化性能  

Isolation and identification of a heterotrophic nitrifying strain of Agrobacterium sp.and its ammoxidation performance

作　　者：邰勇 邓敬轩 任洪艳[1,2,3] 黄振兴 阮文权[1,2,3] TAI Yong;DENG Jingxuan;REN Hongyan;HUANG Zhengxin;RUAN Wenquan(School of Environment and Civil Engineering,Jiangnan University,Wuxi 214122,China;Jiangsu Key Laboratory of Anaerobic Biotechnology,Wuxi 214122,China;Jiangsu Province Water Treatment Technology and Materials Collaborative Innovation Center,Suzhou 215009,China)

机构地区：[1]江南大学环境与土木工程学院,无锡214122 [2]江苏省厌氧生物技术重点实验室,无锡214122 [3]江苏省水处理技术与材料协同创新中心,苏州215009

出　　处：《应用与环境生物学报》2020年第2期325-331,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家水体污染控制与治理科技重大专项(2017ZX07204002);中央高校基本科研业务费专项资金(JUSRP51512)资助。

摘　　要：针对传统污水处理工艺中存在的工艺复杂、脱氮效率低等问题,从江苏无锡市桃花山垃圾渗滤液生化反应池活性污泥中富集、分离及筛选出一株异养硝化菌BT1.通过16S rRNA序列分析,对分离菌株进行鉴定,同时对其异养硝化特性、氨氧化功能基因及氨氧化性能影响因素进行研究.结果显示:分离到的异养硝化菌为农杆菌属Agrobacterium sp..该菌经过32 h培养后,NH_4^+-N去除率为99.77%;TN去除率为96.99%.其中,59.62%TN转换为胞内氮,37.37%TN转化为气态氮;检测不到NO_3^--N和NO_2^--N的积累.结合氨单加氧酶基因(amo A)的PCR成功扩增,进一步证明了BT1菌株具有氨氧化能力.单因子试验结果显示,在温度为30℃、C/N为10-15、pH为7.0-9.0、转速为120-160 r/min的条件下,菌株均能去除98.51%以上NH_4^+-N,体现出良好的氨氧化性能.BT1菌株能够适应较宽的氨氮负荷,在高氨氮浓度(500和1 000 mg/L)下生长良好且NH_4^+-N去除率均超过64.69%.本研究表明BT1菌株具有高效的异养硝化性能及优异的氨氮耐受性,具有进一步处理高浓度氨氮废水的应用前景.(图9参39)Traditional wastewater treatment processes are complicated and nitrogen removal efficiency is low.In this study,a heterotrophic nitrifying bacterium was enriched,isolated,and screened from activated sludge of a landfill leachate biochemical reaction tank located in Taohuashan,Wuxi City,Jiangsu Province.The strain was identified by morphological observation and 16S rRNA sequence analysis.The heterotrophic nitrification characteristics,ammonia oxidation gene function,and influencing factors of heterotrophic nitrification properties were studied.The results showed that the isolated heterotrophic nitrifying bacterium was Agrobacterium sp.and the NH4+-N removal rate reached 99.77%after 32 h incubation.The total nitrogen(TN)removal rate was 96.99%,of which 59.62%of TN was converted to cell growth as internal nitrogen and 37.37%of TN was converted to gaseous nitrogen.The accumulation of NO3--N and NO2--N was not detected.Moreover,the ammonia monooxygenase gene was successfully amplified.The ability of ammonia oxidation for the BT1 strain was proven.The single factor experiment results showed that the strain could remove more than 98.51%of NH4+-N under conditions of 30℃,C/N 10–15,pH 7.0–9.0,and 120–160 r/min,which showed the good ammonia oxidation performance of the strain.The ammonia nitrogen tolerance of the BT1 strain was better and grew well with 500 and 1000 mg/L ammonia nitrogen.The NH4+-N removal rate exceeded 64.69%.The high-efficiency heterotrophic nitrification performance and excellent ammonia nitrogen tolerance makes the BT1 strain ideal for use in wastewater treatment processes.

关 键 词：异养硝化菌 农杆菌 氨氧化 氨单加氧酶 

分 类 号：X172[环境科学与工程—环境科学] X703