通过脑干听觉诱发电位观察电项针治疗脑干缺血大鼠即时效应  

Immediate Effect Observation of Electric Acupuncture on Brainstem Ischemic Rats Through Brainstem Auditory Evoked Potential

作　　者：刘勇[1] 魏鑫甜 LIU Yong;WEI Xintian(First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150036,Heilongjiang,China;Heilongjiang University of Chinese Medicine,Harbin 150036,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150036 [2]黑龙江中医药大学,黑龙江哈尔滨150036

出　　处：《辽宁中医药大学学报》2020年第3期8-11,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：黑龙江中医药大学研究生创新科研项目(yjscx2018031);黑龙江省博士后资助项目(LBH-Z11011)。

摘　　要：目的采用电凝椎动脉的方法建立脑干缺血大鼠模型,通过转棒实验、Bederson评分、脑干听觉诱发电位检测观察电项针对脑干缺血大鼠模型的即时疗效。分析通过针灸及电项针对脑干缺血大鼠进行即刻治疗时脑干听觉诱发电位(BAEP)的变化情况。方法选取清洁级健康雄性SD成年大鼠60只,经常规适应性喂养及转棒实验适应性训练1周后开始正式实验。将大鼠随机分为4组,分别为假手术组、模型组、电项针组、针刺组。记录各组大鼠治疗前后于转棒上停留时间及Bederson评分情况。在造模结束24 h后(治疗前)进行第1次BAEP检测,首次治疗结束后立即进行第2次BAEP检测,比较4组大鼠Ⅰ、Ⅲ、V波振幅及Ⅰ、Ⅲ、Ⅴ波的波峰潜伏期。结果相较于假手术组,模型组、针刺组、电项针组3组大鼠造模24 h后于转棒上停留时间明显减少(P<0.01),Bederson评分明显增高;即刻治疗后,电项针组相较模型组及针刺组于转棒上停留时间明显增加(P<0.05),Bederson评分明显增高;模型组、针刺组、电项针组大鼠造模24 h后进行BAEP检测,Ⅲ、Ⅴ波振幅明显压低,Ⅲ、Ⅴ波峰潜伏期波峰明显延长(P<0.01),假手术组不出现变化,Ⅰ波无明显变化(P>0.05),即刻治疗后电项针组较针刺组及模型组大鼠Ⅲ、Ⅴ波振幅抬高(P<0.05),Ⅲ、Ⅴ波峰潜伏期缩短(P<0.05),Ⅰ波无明显变化(P>0.05)。结论电项针即刻治疗可明显改善脑干大鼠的脑干血液循环,其效果优于即刻针刺治疗。Objective To establish a rat model of brainstem ischemia by electrocoagulation of vertebral artery.To observe the immediate effect of electro-acupuncture on brainstem ischemia rat model by rotatory rod test and Bederson score brainstem auditory evoked potential test.To analyze the changes of brainstem auditory evoked potential(BAEP)in rats with brainstem ischemia treated by acupuncture and electroacupuncture.Methods Sixty healthy male SD adult rats of clean grade were selected.Regular adaptive feeding and adaptive training of rotary rod experiment were conducted one week later.The rats were randomly divided into four groups:sham operation group,model group,electro-nape acupuncture group and acupuncture group.The retention time on the rotating rod and Bederson score were recorded before and after treatment.The first BAEP was detected 24 hours after the end of modeling(before treatment),and the second BAEP was detected immediately after the end of the first treatment.The amplitudes of waveⅠ,ⅢandⅤand peak latency of waveⅠ,ⅢandⅤwere compared among the four groups.Results Compared with sham operation group,the retention time on rotating rod in model group,acupuncture group and electro-nape acupuncture group decreased significantly 24 hours after modeling(P<0.01),and the Bederson score increased significantly.After immediate treatment,the retention time on rotating rod in electro-nape acupuncture group increased significantly compared with model group and electro-nape acupuncture group(P<0.05),while the Bederson score increased significantly in model group,electro-nape acupuncture group and electro-nape acupuncture group.BAEP test showed that the amplitudes of waveⅢandⅤwere significantly decreased,the latency of waveⅢandⅤwas significantly prolonged(P<0.01),and there was no change in sham-operated group,but no change in waveⅠ(P>0.05).After immediate treatment,the amplitude of waveⅢandⅤin EA group increased(P<0.05),the latency of waveⅢandⅤshortened(P<0.05),and the waveⅠdid not change signi

关 键 词：脑干听觉诱发电位 脑干缺血大鼠 电项针 即刻治疗 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R245[医药卫生—临床医学]