组织工程三维多孔骨支架内部微流体流场研究  

Study on microfluidic flow field inside tissue engineering three-dimensional porous bone scaffold

作　　者：赵士明[1,2,3] 李文雷 赵静一 郭锐[1,2] 高远[4] 卢子帅 Zhao Shiming;Li Wenlei;Zhao Jingyi;Guo Rui;Gao Yuan;Lu Zishuai(Hebei Provincial Key Laboratory of Heavy Machinery Fluid Power Transmission and Control,Yanshan University,Qinhuangdao 066004;Key Laboratory of Advanced Forging&Stamping Technology and Science(Yanshan University),Qinhuangdao 066004;Department of Mechanical Engineering,Tangshan Polytechnic College Mechanical Engineering,Tangshan 063299;Institute of Traumatic Surgery,The Second Hospital of Tangshan,Tangshan 063001)

机构地区：[1]燕山大学河北省重型机械流体动力传输与控制实验室,秦皇岛066004 [2]先进锻压成形技术与科学教育部重点实验室(燕山大学),秦皇岛066004 [3]唐山工业职业技术学院机械工程系,唐山063299 [4]河北省唐山市第二人民医院创伤外科研究所,唐山063001

出　　处：《高技术通讯》2020年第5期518-525,共8页Chinese High Technology Letters

基　　金：国家自然科学基金(51675461,11673040);河北省科技厅科技计划项目(16211303)资助。

摘　　要：组织工程支架作为培养组织的载体,能为组织提供各种微环境,是决定组织培养能否成功的关键。针对骨组织工程体外培养对支架内部微流体流场的要求,设计了包括仿生结构和螺旋结构的4种骨组织工程三维多孔支架。基于Navier-Stokes方程建立了骨支架内部流场的数学模型,利用ANSYS Fluent对骨组织工程支架内部营养液和骨细胞流动状态进行了数值模拟,分别得到了4种骨组织工程支架流场的压力分布云图、速度分布云图和剪应力变化曲线。研究结果表明,仿生结构骨支架主管道与哈佛管、浮克曼管的流速相差很大,而螺旋结构支架流体速度分布均匀;分析了壁面流体剪应力分布情况,仿生结构骨支架壁面流体剪应力偏大,且分布不均匀,而螺旋结构支架剪应力偏小,但分布均匀;通过调整边界条件与优化内部结构,流体压力、流速更均匀,流体剪应力达到0.2~0.3 Pa之间,满足人体组织生长的需要。这对骨组织工程支架的设计与应用提供一定的指导作用。Tissue engineering scaffolds,as carriers of cultured tissues,can provide various microenvironments for tissues and are the key to determining the success of tissue culture.Aiming at the requirements of micro-fluid flow field in the internal culture of bone tissue engineering,four kinds of bone tissue engineering three-dimensional porous scaffolds including bionic structure and spiral structure are designed.The mathematical model of the internal flow field of the bone scaffold is established based on the Navier-Stokes equation.The flow of nutrient solution and bone cell flow in the bone tissue engineering scaffold are numerically simulated by ANSYS Fluent,and the pressure of the flow field of the four bone tissue engineering scaffolds is obtained,and the pressure distribution cloud map,velocity distribution cloud map and shear stress variation curve of the four bone tissue engineering scaffold flow fields are obtained.The results show that the flow velocity of the main pipeline of the bionic structural bone scaffold is very different from that of the Haversian canal and the Volkmann’s canal,and the fluid velocity distribution of the spiral structure scaffold is even.The shear stress distribution of the wall fluid is analyzed,and the fluid shear stress of the wall of the bionic structure is larger and the distribution is not uniform,while the shear stress of the spiral structure is smaller,but the distribution is even.By adjusting the boundary conditions and optimizing the internal structure,the fluid pressure and flow rate are more uniform,and the fluid shear stress reaches between 0.2 and 0.3 Pa,which satisfies the needs of human tissue growth.This will provide some guidance for the design of bone tissue engineering scaffolds.The conclusions of this work provide some guidance for the design and application of bone tissue engineering scaffolds.

关 键 词：骨组织工程支架 流场 微流动 流体剪应力 

分 类 号：G63[文化科学—教育学]